丹参多酚酸盐对ApoE基因敲除小鼠血清脂质的干预作用

目的:观察中药丹参多酚酸盐对高脂饮食ApoE-/-小鼠脂质代谢的影响,探讨丹参多酚酸盐抗动脉粥样硬化作用机制。方法:将4周龄雄性C57BL/6J ApoE-/-小鼠喂食高脂饮食,随机分为4组。模型组腹腔注射生理盐水、丹参多酚酸盐干预分为低剂量组(60 mg/kg)、中剂量组(120 mg/kg)和高剂量组(240 mg/kg)腹腔注射丹参多酚酸,另设C57BL/6J野生型小鼠为对照组。于24周末时处死各组小鼠,留取血清,检测各组小鼠血清总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)水平。结果:与对照组比较,ApoE-/-鼠血清TC、LDL-C均明显升高(P<0.05),表明敲除ApoE基因能导致血脂代谢紊乱。应用丹参多酚酸盐后,各干预组TC、LDL-C水平均明显降低(P<0.05),但TG和HDL-C水平无明显改变,中、高剂量组尤为显著。结论:丹参多酚酸盐可降低ApoE-/-小鼠血清TC和LDL-C水平,可能是其抗动脉粥样硬化的作用机制之一。

Effects of salvianolate on serum lipid levels in ApoE-/- mice

Objective:To investigate the anti-atherosclerotic effects of salvianolate(Sal),a Chinese medical herb,on serum lipid levels in Apo E-/-mice fed with high fat diet.Methods:Twenty-four C57BL/6J Apo E-/1 mice(4-week old male) were randomly divided into four groups according to intrapertoneal injection of saline(Saline group),60mg/kg(low-dose group),120 mg/kg(middle-dose group),or 240mg/kg(high-dose group) of Sal.Another five C57BL/6J wild mice served as normal controls.At the end of 24 weeks,all animals were sacrificed,and serum levels of total cholesterol(TC),triglyceride(TG),high-density lipoprotein(HDL-C),and low-density lipoprotein(LDL-C) were assessed by a RX-2000 Automatic biochemical analyzer.Results:Compared with normal controls,serum levels of TC and LDL-C were significantly higher in the Apo E-/-mice.After treatment with Sal,serum levels of TC and LDL-C were decreased,which was more obvious in the middle-and high-dose groups.Serum levels of TG and HDL-C were not significantly changed.Conclusion:Sal can decrease serum levels of TC and LDL in Apo E-/1 mice,suggesting its anti-atherosclerotic effects.