| 高效液相色谱法测定复方丹参输卵管灌注液中丹参素含量 |
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| 引用本文： | 杨辉,刘佩坚,苏健芬,李寅,鲁琳,陈汉威.高效液相色谱法测定复方丹参输卵管灌注液中丹参素含量[J].今日药学,2012,22(6):350-352. |
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| 作者姓名： | 杨辉 刘佩坚 苏健芬 李寅 鲁琳 陈汉威 |
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| 作者单位： | 广州市番禺区中心医院,广东广州,511400 |
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| 摘 要： | 目的 建立高效液相色谱法测定复方丹参输卵管灌注液中丹参素含量.方法 色谱柱:Inertsil C18柱(4.6 mm × 250 mm,5 μm);流动相:甲醇-1％冰醋酸,梯度洗脱；流速:1.0ml/min；检测波长:280 nm.结果 丹参素检测浓度在8.5～42.5 μg/ml范围内与峰面积呈良好的线性关系(0.999 9);平均加样回收率为98.36％,RSD为1.02％.结论 该方法简单、灵敏、准确,可用于复方丹参输卵管灌注液的质量控制.
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| 关 键 词： | 复方丹参输卵管灌注液 丹参素 高效液相色谱法 含量测定 |
Determination of Danshensu in Compound Danshen Tubal Perfusion by HPLC Assay |
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| YANG Hui
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LIU Pei-jian
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SU Jian-fen
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LI Yin
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LU Lin
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CHEN Han-wei.Determination of Danshensu in Compound Danshen Tubal Perfusion by HPLC Assay[J].Pharmacy Today,2012,22(6):350-352. |
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| Authors: | YANG Hui LIU Pei-jian SU Jian-fen LI Yin LU Lin CHEN Han-wei |
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| Institution: | (Panyu Central Hospital,Guangzhou,Guangdong 511400,China) |
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| Abstract: | Objective To establish an HPLC method for the determination of danshensu in compound danshen tubal perfusion.Methods Inertsil C18(4.6 mm×250 mm,5 μm) column was gradient eluted with mobile phase consisted of methanol-1% glacial acid(10∶90) at flow rate of 1.0 ml/min.And the detection wavelength was set at 280 nm.Results The linear range of danshensu was 8.5～42.5 μg/ml(r=0.999 9) with an average recovery of 98.36%(RSD=1.02%,n=6).Conclusion It is a simple,rapid and accurate assay which could be applied to the quality control of Compound danshen Tubal Perfusion. |
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| Keywords: | Compound Danshen tubal perfusion danshensu HPLC determination |
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