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| 腺苷蛋氨酸对L02细胞尿苷二磷酸葡萄糖醛酸转移酶表达的影响 | |
| 引用本文： | 司徒冰,崔其亮,粱淑文,梅峥嵘.腺苷蛋氨酸对L02细胞尿苷二磷酸葡萄糖醛酸转移酶表达的影响[J].今日药学,2012,22(6):324-325,331. |
| 作者姓名： | 司徒冰 崔其亮 粱淑文 梅峥嵘 |
| 作者单位： | 1. 广州医学院第三附属医院药学部,广东广州,510150 2. 广州医学院第三附属医院儿科,广东广州,510150 3. 广州医学院第三附属医院广州医学院,广东广州,510150 |
| 基金项目： | 2011年广东省医院药学研究基金 |
| 摘 要： | 目的探讨腺苷蛋氨酸(S-adenosyl-L-methionine,SAMe)对尿苷二磷酸葡萄糖醛酸转移酶(Uridine Diphosphate Glucuronsyltrans-ferase,UGT)表达的调节作用。方法体外培养L02细胞加入药物分别处理0～72 h后应用RT-PCR法观察SAMe对UGT mRNA的作用。结果 0.5 mmol/L的SAMe在24～72 h可上调UGTmRNA的表达,0.1～1 mmol/L的SAMe都能上调UGTmRNA的表达,但0.5mmol/L的作用最强,强于苯巴比妥。结论 SAMe可以上调UGTmRNA的表达,从而促进胆红素代谢。 |
| 关 键 词： | 腺苷蛋氨酸 尿苷二磷酸葡萄糖醛酸转移酶 表达 |
Effects of S-adenosyl-L-methionine on Expression of Uridine Diphosphate Glucuronsyltransferase in L02 Cells |
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| SITU Bing , CUI Qi-liang , LIANG Shu-wen , MEI Zheng-rong.Effects of S-adenosyl-L-methionine on Expression of Uridine Diphosphate Glucuronsyltransferase in L02 Cells[J].Pharmacy Today,2012,22(6):324-325,331. | |
| Authors: | SITU Bing CUI Qi-liang LIANG Shu-wen MEI Zheng-rong |
| Institution: | 1(1.Department of Pharmacy,2.Department of Pediatrics,the Third Affiliated Hospital of Guangzhou Medical College,3.Graduate 2009,Guangzhou Medical College,Guangzhou,Guangdong 510150,China) |
| Abstract: | Objective To detect the function of S-adenosyl-L-methionine(SAMe) on the expression of uridine diphosphate glucuronsyltransferase(UGT).Methods L02 cells were cultured,and they were treated by drugs from 0 to 72 h,respectively.UGTmRNA was detected by RT-PCR.Results SAMe was able to enhance the expression of UGT mRNA from 24 to 72 h.UGTmRNA was regulated by 0.1～1 mmol/L SAMe,and effect of 0.5 mmol/L SAMe was better than phenobarbital.Conclusions SAMe was able to increase the expression of UGTmRNA in L02 cells and promote the metabolism of bilirubin. |
| Keywords: | S-adenosyl-L-methionine Uridine Diphosphate Glucuronsyltransferase expression |
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