HPLC法测定中成药和保健食品中违法添加的6种肾上腺皮质激素

目的 建立测定中成药和保健食品中6种肾上腺皮质激素(醋酸地塞米松、醋酸泼尼松龙、醋酸氢化可的松、醋酸泼尼松、醋酸可的松、倍他米松)的高效液相色谱法.方法 样品经提取浓缩后,以C18柱色谱柱(4.6 mm ×200 mm,μm)分离；流动相为乙腈-水(40∶60)；流速为1 ml/min；紫外检测波长为240nm.结果 6种成分均能良好地提取分离和测定,在进样量20 ～2 000 ng范围内,含量与峰面积线性关系良好(r≥0.999 8),加标回收率为75％～110％.结论 本法简便快速准确,可用于对中成药或保健食品中违法添加的6种肾上腺皮质激素的定性筛查和含量测定.

Determination of 6 Adrenal Corticostreroids Illicitly added in Chinese Traditional Medicine or Health-care Foods by HPLC

Objective To establish a HPLC method to determine 6 adrenal corticostreroids(dexamethasone acetate,prednisolone acetate,hydrocortisone acetate,prednisone acetate,cortisone acetate,betamethasone),which were illicitly added in Chinese traditional medicines or health-care foods.Methods The C18 column(4.6 mm×200 mm,5 μm) was adopted for isolation;acetonitrile-water(40∶60) was used as mobile phase;and UV absorbance was set at 240 nm as detecting wavelength.Results Six adrenal corticostreroids were well resolved and determined.The correlation coefficients of all detection curves were ≥0.999 8 within the injecting quantity range of 20~2 000 ng.The spiked recoveries were 75%~101%.Conclusion The method is simple,efficient,accurate,which can be fully used to determining the 6 adrenal corticostreroids illicitly added in Chinese traditional medicines or health-care foods.