1848例妊娠中期羊水细胞染色体核型分析

目的探讨羊水细胞染色体核型分析在妊娠中期产前诊断中胎儿染色体病的必要性和安全性。方法对妊娠19～23周的符合产前指征的孕妇在B超引导下行羊膜腔穿刺术,抽取羊水15～20ml,对羊水中胎儿脱落细胞进行培养、制片、显带,并分析染色体核型。结果在1848例孕妇的羊水染色体检查结果中,异常核型62例,胎儿染色体异常率为3.35%,其中唐氏高风险919例,异常29例,异常检出率3.16%,18-三体高风险128,异常4例,异常检出率3.15%,NTD高风险22例,异常1例,异常检出率4.54%,高龄736例,异常24例,异常检出率3.26%,不良孕产史40例,异常1例,异常检出率2.50%,超声检查淋巴水囊瘤4例,异常3例,异常检出率75.00%。结论在目前技术阶段,羊水细胞染色体培养和核型分析可显著减少染色体病患儿的出生,在产前诊断中有着不可替代的作用。

1848 example pregnancy intermediate stage amniotic fluid cell chromosome nuclear type analysis

Objective：The discussion amniotic fluid cell chromosome nuclear type analysis the necessity and the security which the embryo chromosome gets sick in the pregnancy intermediate stage pre-natal diagnosis.Methods：To the pregnancy 19～23 week-long the pregnant woman who tallies pre-natal refers drafts in the B ultra guidance downward amniotic cavity puncture method,extraction amniotic fluid 15～20ml,the embryo falls off the cell to the amniotic fluid in to carry on the raise,the movie-making,reveals the belt,and analyzes the chromosome nuclear type.Results：In 1848 example pregnant woman′s amniotic fluid chromosome inspection result,the unusual nuclear 62 examples,the embryo chromosome rate of abnormality is 3.35%,in which Tang high risk 919 examples,exceptionally 29 examples,exceptionally pick out rate 3.16%,18-three body high risks 128,exceptionally 4 examples,exceptionally pick out rate the 3.15%,NTD high risk 22 examples,exceptionally 1 example,exceptionally picks out rate 4.54%,The advanced age 736 examples,exceptionally 24 examples,exceptionally pick out rate 3.26%,is pregnant not good produces the history 40 examples,exceptionally 1 example,exceptionally picks out rate 2.50%,the supersonic inspection lymph hygroma 4 examples,exceptionally 3 examples,exceptionally pick out rate 75.00%.Conclusion：In the present technology stage,the amniotic fluid cell chromosome raise and the nuclear analysis may obviously reduce the chromosome sickness birth,has the function in the pre-natal diagnosis which cannot be substituted.