| 土槿皮乙酸体外对前列腺癌PC-3细胞增殖的抑制作用及机制 |
| |
| 引用本文： | 程文龙,李晨光,马武男,张志龙,冉金生,苏书光. 土槿皮乙酸体外对前列腺癌PC-3细胞增殖的抑制作用及机制[J]. 中药药理与临床, 2011, 0(1): 9-13 |
| |
| 作者姓名： | 程文龙 李晨光 马武男 张志龙 冉金生 苏书光 |
| |
| 作者单位： | 辽宁医学院附属第一医院;北京市通州区老年病医院; |
| |
| 摘 要： | 目的:研究土槿皮乙酸(PAB)对人雄激素非依赖性前列腺癌(AIPC)PC-3细胞系增殖的抑制作用及其可能机制。方法:体外培养AIPC PC-3细胞,以不同浓度PAB进行处理一段时间后,再用MTT法测定PAB对PC-3细胞的生长抑制情况;用台盼蓝染色计数活细胞,用吉姆萨染色法检测凋亡细胞,进一步用Hoechst33342染色进行凋亡确认;用PI染色法检测PAB对PC-3细胞周期的影响;用考马斯亮兰G-250染色法检测PAB对细胞质蛋白的影响;用荧光双染法检测细胞微管蛋白表达。结果:PAB明显抑制体外培养的PC-3细胞生长,其抑制率在一定浓度和时间范围内呈依赖关系,作用36h的IC50值为6.20μM。PAB可以通过诱导PC-3细胞凋亡而抑制期增殖,凋亡细胞的数量随PAB浓度的增加而增加。PAB能明显增加PC-3细胞时G2期细胞的比例,G1期和S期细胞数减少,细胞周期的变化与PAB浓度相关。PAB可以减少PC-3细胞胞质内蛋白含量,抑制微管蛋白形成。结论:PAB可以抑制AIPC PC-3细胞增殖,阻滞细胞于G2/M期(M晚期),呈浓度依赖性;机制可能与PAB降低胞质蛋白量,抑制微管蛋白形成有关。
|
| 关 键 词： | 土槿皮乙酸 PC-3细胞 微管蛋白 |
Inhibitions of pseudolaric acid B on prostate carcinoma PC-3 cells and its mechanism |
| |
| Cheng Wenlong ,Li Chenguang ,Ma Wunan ,Zhang Zhilong ,Ran Jinsheng ,Su Shuguang. Inhibitions of pseudolaric acid B on prostate carcinoma PC-3 cells and its mechanism[J]. Pharmacology and Clinics of Chinese Materia Medica, 2011, 0(1): 9-13 |
| |
| Authors: | Cheng Wenlong Li Chenguang Ma Wunan Zhang Zhilong Ran Jinsheng Su Shuguang |
| |
| Affiliation: | Cheng Wenlong 1,2,Li Chenguang 1,Ma Wunan 2,Zhang Zhilong 2,Ran Jinsheng 2,Su Shuguang 2 (1 The first Affiliated Hospital of Liaoning Medical University,Jinzhou 121001,2 Tongzhou Geriatric Hospital,Tongzhou 101100) |
| |
| Abstract: | Objective: To investigate the inhibitory effect of pseudolaric acid B(PAB) on the androgen-independent human prostate carcinoma(human AIPC) PC-3 cells and the possible mechanism of the action.Methods: AIPC PC-3 Cells were cultured in vitro,and were treated with various concentrations of pseudolaric acid B for some hours.Growth inhibition by pseudolaric acid B was analyzed by MTT assay.Typan blue staining was used to count the number of necrotic cells.The apoptotic cells were detected using Giemsa staining,a... |
| |
| Keywords: | pseudolaric acid B(PAB) PC-3 cells tubulin |
| 本文献已被 CNKI 等数据库收录！ |
|
