金黄地鼠颊囊癌前病变癌变的综合监测研究--DNA、SPF、AgNOR、PCNA及APO的研究

目的评价ＤＮＡ含量（ＤＩ）、倍体、Ｓ期细胞比例（ＳＰＦ）、银染核仁形成区（ＡｇＮＯＲ）、增殖细胞核抗原（ＰＣＮＡ）及细胞凋亡指数（ＡＰＯ）在口腔粘膜癌前病变癌变监测中的价值及临床应用意义。方法应用流式细胞仪（ＦＣＭ）、免疫组化等方法对ＤＭＢＡ致地鼠颊囊粘膜癌前病变及癌变过程中的ＤＮＡ含量、倍体、ＳＰＦ、ＡｇＮＯＲ及ＡＰＯ进行定量检测。结果在Ⅰ—Ⅳ组间（Ⅰ组为正常颊囊粘膜、Ⅱ组为上皮单纯增生、Ⅲ组为上皮异常增生、Ⅳ组为癌肿组）ＤＮＡ含量、异倍体出现率、ＳＰＦ、ＡｇＮＯＲ计数及ＰＣＮＡ表达依次递增;ＡＰＯ指数随Ⅰ—Ⅲ组依次升高,Ⅳ组时下降。多元逐步判别分析,建立判别函数方程,回代判别准确率为９０％。结论本实验结果提示综合监测为口腔粘膜癌前病变癌变的细胞动力学的动态规律的观察提供有益的资料,并对进一步从分子生物学水平研究癌前病变及其癌变将具有重要价值。

Combinationof cell proliferation and apoptosis to predict malig nant potential of Golden Hamster cheek pouch premalignancy

Objective Combination of cell proliferation and apoptosis to predict malignant potential of DMBA-induced Goledn Hamster cheek pouch premalignancy.Methods DMBA-induced golden hamster cheek pouch carcinogenesis model was done.There were four histological types of the model inclouding normal,hyperplasia,dysplasia and carcinoma.DNA Index(DI),SPF,PCNA,AgNOR and apoptosis were detected by flow cytometry(FCM),immunohistochemical and histochemical methods.Results DI,SPF,PCNA and AgNOR indices were increased from normal to premalignancy but decreased after carcinogenesis.Then the discriminant function equation was found using these indices at 90.01 percent of backsubstitution accuracy.Conclusion The indices of DI,SPF,PCNA,AgNOR and apoptosis can reflect the cellular dynaic changes of Hamster cheek pouch carcinogenesis.They revealed the incipient celluar alterations which were not evident in routine pathological examine and contributed to the characterization of different states of carcinogenesis in this model.