| 结核分枝杆菌38-KDa抗原酶联免疫测定结核抗体的方法建立 |
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| 引用本文： | 胡忠义,郭春祥,许成文,李建林. 结核分枝杆菌38-KDa抗原酶联免疫测定结核抗体的方法建立[J]. 上海预防医学, 1998, 0(10) |
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| 作者姓名： | 胡忠义 郭春祥 许成文 李建林 |
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| 作者单位： | 上海市结核病防治中心!200031(胡忠义，许成文)，上海晶莹生物技术公司(郭春祥，李建林) |
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| 摘 要： | 目的：建立检测结核抗体的酶联免疫吸附试验，并探讨方法的最佳测定条件。方法：采用结核分枝杆菌38—KDa基因重组抗原包被固相载体进行酶联免疫测定。结果：170份血清结核抗体测定，敏感性为74．0％，特异性为95．7％；Omega试剂的敏感性为68．0％，特异性为97．1％。两种38－KDa重组抗原的测定结果差异无显著性（P＞0．05）。结论：38一KDa。抗原性能达到或接近Omega产品.本法灵敏、特异，可作为结核病的辅助诊断方法之一。
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| 关 键 词： | 抗原 结核分枝杆菌 基因工程 免疫测定 抗体 |
Establishment of enzyme-immunoassay for the detection of tubercle antibodies with 38 - KDa antigen ofM. tuberculosis |
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| Hu Zhongyi. Establishment of enzyme-immunoassay for the detection of tubercle antibodies with 38 - KDa antigen ofM. tuberculosis[J]. Shanghai Journal of Preventive Medicine, 1998, 0(10) |
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| Authors: | Hu Zhongyi |
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| Abstract: | 0bjective: Establish enzyme - immunoassay for the detection of tubercle antibodies and describeits optimal conditions. Methods:With recombinant 38 - KDa antigen of M. tuberculosis coating soid carrier.Results: The sensitivity and specificity of this method was 74. O % and 95. 7 %, while the Omega reagent was68. 0 % and 97. 1 % respectively by detection of l70 sera samples. There was no significantly differences in re-suIts by two reagents (P >0. O5). Conclusion: According to its sensitity and specificity value, this method canserve as supplementary method f0r the diagnosis of tuberculosis. |
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| Keywords: | Antigen Mycobacterium tuberculosis Genetic engineering Immunoassay Antibody |
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