头穴电针对脑出血大鼠脑组织GLUT-1及共基因表达的影响

目的：研究大鼠脑出血后血肿周围组织葡萄糖载体蛋白-1（GLUT-1）、GLUT-1mRNA表达的动态变化规律及头穴透刺治疗作用。方法：将雄性Wistar大鼠144只随机分为假手术组、模型组、针刺组，每组又分为24h、72h、168h三个小组，采用胶原酶／肝素联合注入法制备脑出血大鼠模型。针刺组给予百会透太阳穴电针治疗，按指定时间断头取脑，取血肿周围3mm范围内组织，分别采用免疫组织化学法检测GLUT-1和RT-PCR法检测GLUT-1mRNA表达的变化。结栗：脑出血后，模型组三个时间点均见大部分GLUT-1阳性微血管形态变窄、皱缩、闭塞，越靠近病灶越严重；与假手术组比较，模型组GLUT-1及GLUT-1mRNA表达24h时失代偿性升高（P〈0．05），72h、168h时失代偿性降低（P〈0．05）。针刺组：仅在24h时血肿周围出现了轻微的管腔狭窄，之后大部分血管表现为管腔呈圆形扩张：GLUT-1与GLUT-1mRNA表现相同的变化趋势，即与假手术组比较，24h时变化不明显，72h、168h时出现降低（P〈0．05），且各时间点与模型组有明显差异（P〈0．05）。结论：头穴电针能扩张血管，改善供血，减轻血管内皮细胞的损伤，故能推迟GLUT-1及GLUT-1mRNA病变的发生，延长治疗时间窗。

Effects of Scalp Electroacupuncture on Cerebral GLUT-1 and GLUT-1 mRNA Expressions in Intracerebral Hemorrhage Rats

Objective： To study the dynamic changes of GLUT-1 and GLUT-1 mRNA expressions of cerebral tissues in intracerehral hemorrhage rats and the acupuncture＇s effects on them. Methods： 144 Wistar rats were divided into 3 groups： sham group, model group, and acupuncture group. Every group was subdivided into three groups in time （24h, 72h, 168h）. The rat model of intracerehral hemorrhage was made with Collagense VII/heparini infusion, and treated by electro-acupuncture at acu- points Baihui （GV20） through Taiyang （EX-HN1）. Immunohistochemical method was used to observe the expressions of GLUT-1 and RT-PCR method to detect GLUT-1 mRNA expression on the perihemotoma. Results： After intracerehral hemorrhage, model group showed narrowing, crimpling and obliteration in majority of GLUT-1-positive micro-vessels in all three times, more severe in nearer loci. In comparison with sham group, the expressions of GLUT-1 and GLUT-1 mRNA compensatorily rose within 24h（P 〈0.05）, and fell in 72h and in 168h （P〈0.05）. In acupuncture group： a few GLUT-1-positive micro-vessels were a bit narrowed in 24h, and they were normal in other times; compared with sham group, the expressions of GLUT-1 and GLUT-1 mRNA changed little in 24h time, and obviously fell in 72h and 168h times, but there were significant differences in comparison with the model group （P〈 0.05）. Conclusion： Scalp electroacupuncture can dilate the vessels, improve blood supply, lessen impairment to endothelial cells, and hence delay GLUT-1 and GLUT-1 mRNA expressions to prolong treatment time window.