| 扶正化瘀方对肝纤维化大鼠间质性基质金属蛋白酶活性的影响 |
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| 引用本文： | 崔红燕,姜哲浩,刘成,刘成海. 扶正化瘀方对肝纤维化大鼠间质性基质金属蛋白酶活性的影响[J]. 上海中医药大学学报, 2003, 17(3): 35-38 |
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| 作者姓名： | 崔红燕 姜哲浩 刘成 刘成海 |
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| 作者单位： | 1. 上海中医药大学博士研究生,上海,200032
2. 上海中医药大学附属曙光医院肝病研究所,上海,200021 |
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| 基金项目： | 上海市教委重点学科建设资助项目,上海市教委曙光计划资助项目 (99SG4 4 ) |
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| 摘 要： | 为探讨扶正化瘀方影响间质性基质金属蛋白酶活性的抗肝纤维化作用机制。采用二甲基亚硝胺腹腔注射4w诱导大鼠肝纤维化模型。随机分为正常组、模型组与治疗组;治疗组成模后以扶正化瘀方灌胃4w。模型组予以等量生理盐水;检测血清肝功能与肝组织羟脯氨酸含量。蛋白印迹法分析肝组织α-平滑肌肌动蛋白、I型胶原、基质金属蛋白酶-13和纤溶酶原激活物抑制剂-1蛋白表达,同位素标记底物凝胶法检测间质性基质金属蛋白酶活性。结果：扶正化瘀方显改善模型大鼠肝功能与肝组织胶原沉积;明显降低肝组织羟脯氨酸含量。抑制肝组织α-SMA、I型胶原蛋白表达;对基质金属蛋白酶-13蛋白表达无影响,但明显降低PAI-1蛋白表达。促进间质性基质金属蛋白酶活性。提示扶正化瘀方可促进肝纤维化大鼠间质性基质金属蛋白酶酶原的激活与活性水平。该作用是扶正化瘀方逆转肝纤维化的重要机制之一。
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| 关 键 词： | 扶正化瘀方 肝纤维化 大鼠 间质性基质金属蛋白酶活性 抗肝纤维化 作用机制 |
| 文章编号： | 1008-861X(2003)03-0035-04 |
Influence of "Health-Supporting and Stasis-Resolving Decoction" on Interstitial Metalloproteinase Activity in Liver Fibrosis Rats |
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| Doctoral Graduate of Shanghai University of Traditional Chinese Medicine CUI Hong-yan JIANG Zhe-hao Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine LIU Cheng LIU Cheng-hai. Influence of "Health-Supporting and Stasis-Resolving Decoction" on Interstitial Metalloproteinase Activity in Liver Fibrosis Rats[J]. Acta Universitatis Traditionis Medicalis Sinensis Pharmacologiaeque Shanghai, 2003, 17(3): 35-38 |
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| Authors: | Doctoral Graduate of Shanghai University of Traditional Chinese Medicine CUI Hong-yan JIANG Zhe-hao Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine LIU Cheng LIU Cheng-hai |
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| Affiliation: | Doctoral Graduate of Shanghai University of Traditional Chinese Medicine CUI Hong-yan JIANG Zhe-hao Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine LIU Cheng LIU Cheng-hai |
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| Abstract: | |
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| Keywords: | Health-Supporting and Stasis-Resolving Decoction" ,liver fibrosis,interstitial metalloproteinase |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
