| 小鼠骨髓组织4种造血生长因子受体cDNA片段的克隆和序列测定 |
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| 引用本文： | 张军权,毛秉智,陈肖华,张浩,董波. 小鼠骨髓组织4种造血生长因子受体cDNA片段的克隆和序列测定[J]. 军事医学科学院院刊, 2002, 26(1): 12-13,17 |
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| 作者姓名： | 张军权 毛秉智 陈肖华 张浩 董波 |
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| 作者单位： | 军事医学科学院放射医学研究所,北京,100850 |
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| 基金项目： | 全军医药卫生科研基金课题资助项目 ( 0 1L0 18) |
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| 摘 要： | 目的：探索大剂量照射后造血生长因子受体基因的表达规律。方法：应用逆转录PCR技术，从小鼠骨髓造血组织中扩增出造血生长因子受体的目的片段，PCR产物与克隆载体连续后转化感受态大肠杆菌，阳性菌落提取质粒后进行序列测定。结果：4种造血生长因子受体片段均扩增成功，PCR产物经测序证实。结论：粒细胞集落刺激因子受体（G－CSFR），促红细胞生长素受体（EPOR）、促血小板生成素受体（c-mpl)和干细胞因子受体（c-kit)cDNA片段的克隆成功，从为整体水平上研究照射后造血生长因子受体的表达规律打下了基础。
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| 关 键 词： | 骨髓 造血生长因子受体 cDNA克隆 基因表达 小鼠 |
| 文章编号： | 1000-5501(2002)01-0012-03 |
cDNA cloning and sequencing of partial sequence of 4 hematopoietic growth factor receptors from mouse bone marrow |
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| ZHANG Jun_Quan,MAO Bing_Zhi,CHEN Xiao_Hua,ZHANG Hao,Dong Bo. cDNA cloning and sequencing of partial sequence of 4 hematopoietic growth factor receptors from mouse bone marrow[J]. Bulletin of the Academy of Military Medical Sciences, 2002, 26(1): 12-13,17 |
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| Authors: | ZHANG Jun_Quan MAO Bing_Zhi CHEN Xiao_Hua ZHANG Hao Dong Bo |
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| Abstract: | Objective:To investigate the expression level of some hematopoietic growth factor receptors after large_dose irradiation.Methods:RT_PCR was used to amplify objective cDNA.PCR products were ligated to pGEM_T vector after gel purification and ligation products were used to transform potent E.coli cells.Positive cells were selected for plasmid purification and sequencing.Results:All 4 genes were successfully cloned.Conclusions:The cloning of partial sequence of G_CSFR,EPOR,c_mpl and c_kit from mouse bone marrow makes analysis of hematopoietic growth factor receptor expression level in vivo possible. |
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| Keywords: | bone marrow hematopoietic growth factor receptor cDNA clone gene expression |
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