急性出血坏死性胰腺炎大鼠胰腺组织结构改变与内毒素血症的关系及纳屈酮的治疗作用

为探讨大鼠急性出血坏死性胰腺炎（ＡＨＮＰ）胰腺组织结构改变与内毒素血症的关系及纳屈酮的治疗作用，用５％牛磺胆酸钠逆行胰胆管注射制成ＡＨＮＰ模型。取Ｗｉｓｔａｒ大鼠１１０只随机分为假手术组（ｎ＝２０）、ＡＨＮＰ组（ｎ＝４５）、纳屈酮治疗组（ｎ＝４５），分别于术后６、１２、２４小时称取胰腺重量，观察ＡＨＮＰ大鼠胰腺组织结构改变，同时测定其血浆淀粉酶和内毒素水平，并与假手术组比较。结果：与假手术组比较，ＡＨＮＰ组血浆淀粉酶、内毒素及胰腺系数升高，在光镜及电镜下可见胰腺损害随时间延长而加重。纳屈酮治疗组与ＡＨＮＰ组相比，其血浆淀粉酶、内毒素及胰腺系数下降，而且胰腺损害减轻。本实验结果提示：ＡＨＮＰ引发内毒素血症，纳屈酮可通过改善胰腺缺血性损害而降低血浆内毒素，延长大鼠生存时间及降低病死率

RELATIONSHIP BETWEEN ENDOTOXEMIA AND STRUCTURAL CHANGE TO PANCREATIC TISSUE AND THERAPEUTIC EFFECTS OF NALTREXONE ON EXPERIMENTAL ACUTE HEMORRHAGIC NECROTIZING PANCREATITIS IN RATS

TO investigate the relationship between endotoxemia and structural change in the pancreatic tissue and therapeutic effects of naltrexone (NTX) on experimental acute hemorrhagic necrotizing pancreatitis in rats. The model of acute hemorrhagic necrotizing pancreatitis (AHNP) of rats was induced by retrograde injection of 5% sodium taurocholate into the pancreatic duct. One hundred and ten Wistar rats were randomly divided into three groups: control group ( n =20),AHNP group ( n =45) and NTX treatment ( n =45) group. The weight of pancreas and plasma levels of amylase and endotoxin were measured as well as changes of the pancreatic histology were examined by light and electric microscope in 6h, 12h, 24h after operation. Results as compared with control groups , amylase and endotoxin in AHNP group were significantly higher in the plasma and the damage to pancreatic tissue was increased in severity as observed with light and electric microscopes in 6h, 12h, 24h after operation. Comparison between NTX treatment groups with AHNP groups demonstrated that amylase and endotoxin were significantly decreased in the plasma, and the damage to pancreatic tissue was reduced in NTX treatment phase. Conclusion these results showed that endotoxemia was induced by AHNP, but NTX decreased the edotoxin level in the plasma and improved the damage of pancreatic tissue. The lethality was significantly lowered and average survival time was prolonged during NTX treatment of AHNP.