Immunohistochemical diagnosis in fine-needle aspiration cytology

This paper reports 25 kinds of polyclonal or monoclonal antibodies by ABC immunohistochemical technique used for 253 cell smears by fine-needle aspiration. The results were: 1. Immunohistochemical diagnosis were classified into 136 metastatic cancers (K ₁₂ ⁺ EMA⁺ CEA+ LCA^?), 92 lymphomas (LCA⁺ k_12? EMA^? CEA^?), 4 mesenchymal tumors (Vimentin⁺), 3 melanomas (S-100⁺NSE+). 15 reactive proliferations (k⁺ λ⁺ CD4 ₄ ⁺ CD8 ₈ ⁺ ) and 3 unspecified. 2. The origin of 70 metastatic cancers were classified into 36 lung (HLC₃-AB⁺), 4 gastrointestinal tract (MG7⁺), 8 thyroid (TGB⁺), 1 prostate (PSA⁺), 3 liver (AFP⁺) and 14 unknown. 3. Immunologic phenotype of 87 lymphomas wereclassified into 66 cases of B- cell, 4 T-cell, 3 hsitocyte, 7 Hodgkin’s diseases and 7 unclear. The above results suggest that immunohistochemical method may be used as a new method of diagnosing and differentiating epithelial and non-epithelial tumors, detecting primary focus of metastatic cncer, differentiating between reactive proliferation adn lymphome and specifying immunologic phenotype of lymphoma in cell smears of fine- needle aspiration.