Requirement of CD28-CD86 co-stimulation in the interaction between antigen-primed T helper type 2 and B cells |
| |
Authors: | Nakajima A; Watanabe N; Yoshino S; Yagita H; Okumura K; Azuma M |
| |
Institution: | Department of Immunology, Juntendo University School of Medicine, Tokyo, Japan. |
| |
Abstract: | The interaction between CD28 and its ligands, CD80 and CD86, is crucial for
an optimal activation of antigen-specific T cells. However, the requirement
of CD80 or CD86 co-stimulation in Th2 cell differentiation and activation
is controversial. Freshly isolated murine CD4+ and CD8+ T cells were
incubated with P815 transfectants expressing a similar level of either CD80
or CD86 in the presence of anti-CD3 mAb. Both CD80 and CD86 co-stimulated
the proliferation of CD4+ and CD8+ T cells at comparable time-kinetics and
magnitude, but CD86 alone was able to co- stimulate IL-4 and especially
IL-10 production in CD4+ T cells. In typical Th2-dependent immune responses
elicited by Nippostrongylus brasillensis infection, the anti-CD86 mAb
treatment but not the anti- CD80 mAb treatment efficiently inhibited
antigen-specific IgE and IgG1 production, which was accompanied with the
reduced IL-4 production. Our results suggest that CD86 co-stimulation plays
a dominant role not only in the primary activation of Th2 cells but also in
the secondary interaction between antigen-primed Th2 cells and B cells.
|
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|