Wnt信号通路靶基因GS mRNA及蛋白在胃癌中的表达

目的: 探讨Wnt信号通路的靶基因GS mRNA及蛋白在胃癌中的表达及其临床意义.方法: 荧光实时定量PCR(FQ-PCR)检测52例胃癌组织及癌旁正常组织GS mRNA表达;免疫组织化学技术(SP法)检测97例胃癌组织、30例癌旁正常组织及10例肠化生组织中GS蛋白表达水平差异.结果: 癌组织和癌旁正常组织GS mRNA表达有显著差异(25.508±5.090 vs 13.001±2.040,P<0.05). 癌组织GS蛋白表达与组织学类型、Lauren分型及淋巴结转移密切相关(χ2= 26.994,54.929,5.173,均P<0.05),与肿瘤大小、TNM分期、远处转移及患者的性别和年龄等无明显相关.结论: GS mRNA和蛋白高表达同胃癌生物学行为密切相关,可能与胃癌的发生、发展及预后有关.

Expression of the new target GS mRNA and protein of Wnt signaling pathway in gastric cancerous tissue

AIM: To investigate the new target of Wnt signaling pathway GS mRNA and protein expression in cancerous tissue from patients with gastric carcinoma and to evaluate its clinic significance. METHODS: Fluorescent quantitative PCR (FQPCR) was used to detect the GS mRNA expression in cancerous tissue and paracancerous normal mucosa from 52 patients with gastric carcinoma. Immunohistochemistry (SP method) was used to detect GS expressions in paraffinembedded gastric carcinoma tissues of 97 cases,paracancerous normal mucosa of 30 cases and 10 intestinal metaplasia tissues.RESULTS: The expression of GS mRNA was significantly higher in cancerous tissue from patients with gastric carcinoma than in normal tissue (25.508 ± 5.090 vs 13.001 ± 2.040,P < 0.05).GS protein expression was closely related to the pathologic parameters,such as histological type,Lauren classification and distant metastasis (χ2= 26.994,54.929,5.173,all P < 0.05). However,there was no significant correlation between GS expression and lymph node metastasis,tumor size,TNM staging,age and gender of patients.CONCLUSION: GS mRNA and protein expression is significantly correlated with the biological behavior of gastric carcinoma. The high expression of GS may be related with the occurrence and progress of gastric cancer.