C 肽对失血性休克大鼠急性肺损伤的保护作用简

目的：探讨C肽复苏对失血性休克大鼠急性肺损伤的保护作用。方法24只雄性Wistar大鼠随机分为3组：假手术组（ Sham组），失血性休克＋乳酸钠林格液复苏组（ Ringer’s lactate ， RL组），失血性休克＋C肽复苏组（ C肽组）。实验结束测定各组大鼠肺组织髓过氧化物酶（ marrow perioxidase ，MPO）活性；采用ELISA法检测各组大鼠血浆中炎症因子白细胞介素-1（interleukin 1， IL-1）、白细胞介素-6（interleukin 6， IL-6）及肿瘤坏死因子-α（tumor ne-crosisfactor， TNF-α）水平，Western blot法分析肺组织核转录因子-κB（ nuclear factor-kappaB ）蛋白表达水平，同时观察肺的组织学改变。结果与Sham组相比，C肽组和RL组肺组织MPO活性、血浆IL-1、IL-6、TNF-α水平显著升高（P＜0．01），而C肽组较RL组则明显下降（P＜0．01）；C肽组与RL组肺组织NF-κB蛋白表达较Sham组增加（P＜0．01），C肽组较RL组明显减少（P＜0．01）。结论 C肽可能通过抑制促炎细胞因子的表达而减轻失血性休克诱发的急性肺损伤。

The effect of C-peptide on acute lung injury after hemorrhagic shock in rats

Objective To investigate the effect of C-peptide on acute lung injury after hemorrhagic shock in rats .Methods Male Wistar rats were randomly divided into 3 groups： sham, Ringer’s lactate（RL） and C-peptide groups.The level of MPO in lung tissue was detemined.Plasma levels of IL-1, IL-6, TNF-αwere determined by ELISA method .The expression of NF-κB was detected by Western blot in lung tissue .Results The level of MPO in lung tissue , and Plasma levels of IL-1,IL-6, TNF-αincerased in C-peptide group and RL group compared with Sham group （P〈0.01）, The level of MPO in lung tissue,and Plasma levels of IL-1,IL-6, TNF-αincerased in C-peptide group decreased more significantly thanin RL group（P 〈0.01）.The expression of NF-κB in lung tissure increased in C-peptide group and RL group comparedwith Sham group（P 〈0.01）, The expression of NF-κB in C-peptide group decreased more significantly than in RL group（P 〈0.01）.Conclusion C-peptide may exert renoprotective effects on acute lung injury after hemorrhagic shock byinhibiting inflammation.