盾叶薯蓣中新甾体皂苷的研究

为了研究盾叶薯蓣的水溶性化学成分, 用溶剂提取、色谱分离等方法从盾叶薯蓣的水溶液中分离到一个新甾体皂苷, 经¹H NMR、¹³C NMR、¹³⁵DEPT、HMQC、HMBC和TOCSY谱鉴定, 该化合物的结构为: (25R)-26- O-(β-D-glucopyranosyl)-furost-5-en-3b, 16, 20, 26-tetraol-22-seco-3-O-b-D-glucopyranosyl-(1→3)-b-D-glucopyranosyl- (1→4)-a-L-rhamnopyranosyl-(1→2)]-b-D-glucopyranoside, 命名为zingiberenin F (1)。该化合物为一新甾体皂苷化合物, 该骨架甾体皂苷在薯蓣科中为首次报道。

Isolation and structure determination of steroidal saponin from Dioscorea zingiberensis

To study the chemical constituents of Dioscorea zingiberensis Wright, the EtOH extract of fresh rhizomes of D. zingiberensis was concentrated and partitioned further to produce petroleum ether-, ethylacetate-, n-butanol- and water-soluble fractions.  The water-soluble fraction was subjected to column chromatography on macro resin AB-8, and the final products were obtained by repeated reversed-phase ODS and MCI gel CHP 20P column chromatography.  Structures of compounds were elucidated by ¹H NMR, ¹³C NMR, ¹³⁵DEPT, HMQC, HMBC and TOCSY spectroscopic analyses.  A new steroidal saponin was isolated, which was identified as (25R)-26-O-(β-D-glucopyranosyl)-furost-5-en-3b, 16, 20, 26-tetraol-22-seco-3-O-b-D-glucopyranosyl-(1→ 3)-b-D- glucopyranosyl-(1→ 4)-a-L-rhamnopyranosyl-(1→2)]-b-D-glucopyranoside.  The compound is a novel skeletally steroidal saponin, named as zingiberenin F (1).  It was reported for the first time from D. zingiberensis Wright.