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| p38MAPK基因对人胶质瘤细胞BT325生长周期的影响 | |
| 引用本文： | 章必成,张智勇,叶菁,李青,杜光祖. p38MAPK基因对人胶质瘤细胞BT325生长周期的影响[J]. 中国肿瘤临床与康复, 2002, 9(6): 16-18 |
| 作者姓名： | 章必成 张智勇 叶菁 李青 杜光祖 |
| 作者单位： | 1. 广州军区武汉总医院肿瘤科,武汉,430070 2. 广州军区武汉总医院普通外科,武汉,430070 3. 第四军医大学病理学教研室,西安,710032 |
| 摘 要： | 目的 研究 p3 8MAPK基因对人多形性胶质母细胞瘤BT3 2 5生长周期的影响。 方法 利用脂质体介导法将p3 8MAPK基因导入BT3 2 5细胞中 ,用免疫细胞化学染色和Western blot检测其在转染前后的表达情况 ,用HE染色、透射电镜和流式细胞仪等研究其对细胞形态和生长周期的影响。结果 pCMV 5 p3 8MAPK组p3 8MAPK蛋白表达阳性 ,细胞形态发生变化 ,G1%增多 ,而S %和G2 %减少 ,并出现凋亡峰。结论 p3 8MAPK基因可影响BT3 2 5细胞的生长周期 ,并诱导BT3 2 5细胞凋亡。 |
| 关 键 词： | p38MAPK 基因转染 胶质瘤 生长周期 |
| 文章编号： | 1005-8664(2002)06-0016-03 |
| 修稿时间： | 2001-10-24 |
The effect of p38MAPK on the cell cycle of human glioma cells BT325 |
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| ZHANG Bi cheng,ZHANG Zhi yong,YE Jing,et al.. The effect of p38MAPK on the cell cycle of human glioma cells BT325[J]. Chinese Journal of Clinical Oncology and Rehabilitation, 2002, 9(6): 16-18 | |
| Authors: | ZHANG Bi cheng ZHANG Zhi yong YE Jing et al. |
| Affiliation: | ZHANG Bi cheng,ZHANG Zhi yong,YE Jing,et al. Department of Oncology,Wuhan General Hospital,Guangzhou Command,PLA,Wuhan 430070,China |
| Abstract: | Objective To study the effect of p38MAPK on the cell cycle of human glioma cells BT325.Methods p38MAPK was transfected into BT325 cells by lipofectin.Expression of p38MAPK was detected by immunocytochemistry and Western blot.HE staining,transmission electron microscopy and flow cytometry were adopted to measure the cell morphology and cell cycle.Results In the pCMV5 p38MAPK group,p38MAPK expressed positively, the cell morphology changed,G1% increased,S% and G2% decreased,and apoptotic peak emerged.Conclusion p38MAPK could affect the cell cycle and induce the apoptosis of BT325 cells. |
| Keywords: | p38MAPK gene transfection glioma cell cycle |
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