PTEN 、MMP27 、VEGF 在骨巨细胞瘤中的表达

 目的 探讨骨巨细胞瘤中的PTEN、MMP-7、VEGF表达与“三结合分类”、病理分级及复发、转移的关系。方法 采用免疫组化法检测65例骨巨细胞瘤标本盯EN、MMP-7、VEGF基因蛋白的表达，分析其与骨巨细胞瘤临床病理分期、分级及复发、转移的关系。结果 PTEN、MMP-7、VEGF的阳性表达率分别为56．95％、72．30％、49．20％；在“三结合分类”组中PTEN的阳性表达率分别为83．3％、68．10％、28．00％，呈下降趋势，良性与中间性组间差异不显著（P=0．271），而中间性与恶性组间差异明显（P=0．001）；而MMP-7阳性表达率分别为39．00％、72．50％、96．00％，VEGF阳性表达率分别为16．67％、54．50％、68．00％，组间差异明显（P=0．031、0．001）；在转移组中PTEN、MMP-7、VEGF的阳性表达率分别为30．00％、100％、85．00％，在无转移组中PTEN、MMP-7、VEGF的阳性表达率分别为68．80％、60．00％、33．3％，两组间差异明显（P=0．003、0．001、0．001）；复发组中PTEN、MMP-7、VEGF的阳性表达率分别为33．30％、93．30％、86．60％，无复发组中PTEN、MMP-7、VEGF的阳性表达率分别为64．00％、66．00％、38．00％，两组间差异明显（P=0．035、0．038、0．001），且PTEN与MMP-7、VEGF负相关（P=0．001、0．020），而与病理分级及年龄、性别无统计学差异。结论 PTEN、MMP-7、VEGF的表达与“三结合分类”有一定的关系，并与转移、复发显著相关，联合检测有助于骨巨细胞瘤的临床评估，对其预后判断具有重要临床意义。

Expressions of PTEN and MMP-7、VEGF in Giant Cell Tumor of Bone (GCT)

Objective 　To evaluate the expressions of PTEN ( phosphatase and tension homologue deleted on chromosome 10) ,MMP-7 (matrix metalloproteinases-7 ,MMP-7) ,VEGF (vascular endothelial growth factor) and their relationships with clinical factors ,pathological types and metastasis ,recurrence of giant cell tumor of bone ( GCT) . Methods 　The expressions of PTEN , MMP-7 , VEGF in 65 cases of human GCT were detected by using immunohistochemical staining. The expressions of PTEN , MMP-7 , VEGF and other clinical fractures were analyzed with the metastasis and recurrence of the tumor. Results 　The expressions of the PTEN ,MMP-7 ,VEGF in GCT were 56. 95 % and 72. 30 % ,49. 20 % , respectively. In the clinical-pathological-X-ray groups , the expressions of PTEN were 83. 30 % ,68. 10 % , 28. 00 % , the positive rate had no significantly difference between the Non2malignant and doutful-malignant groups , P> 0. 05 ( P = 0. 271) , but there were significantly difference between the doutful-malignant and malignant groups , P < 0. 05 ( P = 0. 001) ; the expression of MMP-7 were 39. 00 % ,72. 50 % ,96. 00 % ,and the expression of VEGF were 16. 67 % ,54. 50 % ,68. 00 % , the positive rates of them had significantly difference , P < 0. 05 ( P = 0. 031 ,0. 001) ; the expressions of PTEN ,MMP-7 ,VEGF in the recurrence group were 33. 30 % ,93. 30 % ,86. 60 % , but in the nonrecurrence group were 64. 00 % ,66. 00 % ,38. 00 % ,their expressions were statistically related to the recurrence of GCT , P < 0. 05 ( P = 0. 035 , 0. 038 ,0. 001) ; in the metastasis group , the expressions of PTEN ,MMP-7 ,VEGF were 30. 00 % ,100 % ,85. 00 % ,in the no metastasis group ,the positive rates of PTEN ,MMP-7 ,VEGF were 68. 80 % ,60. 00 % ,33. 30 % ,the positive rates of them had significantly difference , P < 0. 05 ( P = 0. 003 ,0. 001 ,0. 001) ;at the same time , The expression of PTEN in GCT was inversely correlated with the expressions of VEGF and MMP-7 , P <0. 05 ( P = 0. 001 , 0. 02) ; However , the expressions of PTEN 、MMP-7 、VEGF had no statistically significance with the pathological types and sexuality. Conclusion 　The decreased expression of PTEN and increased expressions of MMP-7 、VEGF have significant relationship to the prognosis of GCT. The combined detection of PTEN and MMP-7 may be of important clinical value to evaluate the infilt rative ability and prognosis of GCT.