乙肝病毒DNA与前S1抗原及HBcAg检测的意义

目的探讨乙型肝炎病毒DNA与前S1蛋白及HBcAg在乙肝患者血清中的相关性及临床意义。方法对280例乙肝的血清采用酶联免疫吸附法(EIISA)检测前S1抗原、HBcAg,定量PCR检测HBV-DNA。结果HBV-DNA阳性的130例中HBcAg阳性率为82.30%,前Sl抗原的阳性检出率为79.23%,在前Sl抗原阳性的94例中HBcAg阳性率为17.02%,在前S1抗原的阴性的70例中,HBcAg阳性率为8.57%,两者比较有显著性差异(<0.05)。前Sl抗原与HBcAg阳性患者的HBV-DNA拷贝数大部分在107~l08IU/ml之间,且HBcAg的阳性率明显高于前S1阳性率,当HBV-DNA在l03~106IU/ml时,前Sl的阳性率高于HBcAg的阳性率。结论前S1抗原、HBcAg与HBV-DNA符合率较高,检测前Sl抗原和HbcAg同样具有重要的临床意义。

Clinical Research on Detection of Hepatitis B Virus DNA and Pre-S1 Antigen and HBcAg

Objective To betterunderstand theduplication ofhepatitisBvirus(HBV)andsuggestclinical diagnosis and treatment via comparison of the correlation of HBV-DNA,HBcAg with HBV Pre-Sl antigen.Me-thods For the 280 HBsAg positive patients,measure HBV Pre-Sl antigen with ELISA and HBV-DNA.Results Before treatment to the 280 patients of hepatitis B virus infection,in the 130 HBV-DNA positive,the positive rate of HBcAg was 82.81% and that of HBV Pre-Sl antigen was 79.68%.In the 94 HBV Pre-Sl antigen positive,the positive rate of HBcAg was 17.02%,while in the 70 HBV Pre-Sl antigen negative the positive rate of HBcAg was 8.57%.Conclusion HBV Pre-Sl antigen and HBcAg accompany HBV-DNA.It is clinically significant to detect Pre-Sl antigen and HBcAg in Hepatitis B immunological examination.