| 乙肝病毒X基因参与肝细胞脂肪变性的作用及机制的初步研究 |
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| 引用本文： | 张琴,彭俊,沈薇.乙肝病毒X基因参与肝细胞脂肪变性的作用及机制的初步研究[J].第三军医大学学报,2011,33(23):2466-2470. |
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| 作者姓名： | 张琴 彭俊 沈薇 |
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| 作者单位： | 1. 重庆医科大学附属第二医院消化内科,重庆,400010
2. 凉山州第一人民医院妇产科,四川西昌,615000 |
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| 基金项目： | 国家自然科学基金(30871160); 重庆市自然科学基金(CSTC2008BB5404); 重庆市医学科技计划项目(2008-2-401)~~ |
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| 摘 要： | 目的 探讨沉默HBx基因对HepG2.2.15细胞脂质代谢相关基因表达的影响.方法 设立空白对照组(HepG2.2.15细胞)、阴性对照组(HepG2.2.15细胞转染阴性HK质粒)、shHBx转染组(HepG2.2.15细胞转染shHBx质粒)和HepG2组(HepG2细胞).构建针对HBx基因的shHBx质粒,转染...
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| 关 键 词： | 乙肝病毒X基因 肝细胞脂肪变性 质粒 RNA干扰 |
Preliminary study of the effect and mechanism of hepatitis B virus X gene in hepatic steatosis |
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| Zhang Qin,Peng Jun,Shen Wei.Preliminary study of the effect and mechanism of hepatitis B virus X gene in hepatic steatosis[J].Acta Academiae Medicinae Militaris Tertiae,2011,33(23):2466-2470. |
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| Authors: | Zhang Qin Peng Jun Shen Wei |
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| Institution: | Zhang Qin1,Peng Jun2,Shen Wei1(1Department of Gastroenterology,Second Affiliated Hospital,Chongqing Medical University,Chongqing,400010,2Department of Obstetrics and Gynecology,First People's Hospital of Liangshan Prefecture,Xichang,Sichuan Province,615000,China) |
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| Abstract: | Objective To investigate the effect of hepatitis B virus X(HBx) gene silencing on expression of lipid metabolism related genes in HepG2.2.15 cells.Methods Cells were divided into a blank control group(HepG2.2.15 cells),a negative control group(HepG2.2.15 cells transfected with negative HK plasmids),a shHBx group(HepG2.2.15 cells transfected with shHBx plasmids) and a HepG2 group(HepG2 cells).shHBx plasmid was constructed and transfected into HepG2.2.15 cells using PolyJetTM reagent.Expressions of green fluo... |
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| Keywords: | hepatitis B virus X gene hepatic steatosis plasmid RNA interference |
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