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| 大鼠急性肠梗阻不同时段血浆内毒素和D-乳酸变化 | |
| 引用本文： | 李辉,王骥平,王培戈. 大鼠急性肠梗阻不同时段血浆内毒素和D-乳酸变化[J]. 青岛大学医学院学报, 2011, 47(6): 501-503,506 |
| 作者姓名： | 李辉 王骥平 王培戈 |
| 作者单位： | 1. 青岛大学医学院附属医院急诊内科,山东青岛,266003 2. 青岛市市立医院急诊普外科 3. 青岛大学医学院附属医院急诊普外科 |
| 摘 要： | 目的探讨大鼠急性肠梗阻不同时段血浆内毒素和D-乳酸的变化。方法 Wistar大鼠随机分为正常对照组(n=8)、假手术组(n=32)和肠梗阻组(n=32),分别于术后8、24、72、168h取下腔静脉血,采用偶氮显色法测定血浆内毒素水平,改良分光光度法测定血浆D-乳酸水平。结果肠梗阻组术后8h血浆内毒素水平明显升高,24h有所下降,后又逐渐升高,至术后168h达高峰,各时点比较,差异有显著性(F=52.75,q=2.67～9.64,P<0.05);肠梗阻组各时点血浆内毒素水平均高于假手术组和对照组,差异有显著性(t=3.24～13.36,P<0.05)。肠梗阻组术后8h血浆D-乳酸浓度升高,呈双峰型变化,至术后168h仍明显高于对照组,差异有显著意义(F=106.28,q=5.36～13.17,P<0.05);肠梗阻组各时点血浆D-乳酸水平均高于假手术组和对照组(t=2.57～8.35,P<0.05);肠梗阻组血浆内毒素与D-乳酸水平呈正相关(r=0.632,P<0.05)。结论血浆D-乳酸水平可作为内毒素血症的预警指标,血浆内毒素水平可作为评价肠道屏障功能损伤程度的指标。 |
| 关 键 词： | 肠梗阻 内毒素 D-乳酸 大鼠 |
CHANGES OF PLASMA LEVELS OF ENDOTOXIN AND D-LACTATE IN RATS WITH ACUTE BOWEL OBSTRUCTION |
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| LI Hui,WANG Jiping,WANG Peige. CHANGES OF PLASMA LEVELS OF ENDOTOXIN AND D-LACTATE IN RATS WITH ACUTE BOWEL OBSTRUCTION[J]. Acta Academiae Medicinae Qingdao Universitatis, 2011, 47(6): 501-503,506 | |
| Authors: | LI Hui WANG Jiping WANG Peige |
| Affiliation: | LI Hui,WANG Jiping,WANG Peige(Department of Emergency,The Affiliated Hospital of Qingdao University Medical College,Qingdao 266003,China) |
| Abstract: | Objective To explore the changes the levels of plasma endotoxin and D-lactate in rats with acute bowel obstruction(ABO) at different time points.Methods Wistar rats were randomly divided into normal control group(8 rats),sham-operation(SO) group(32 rats),and ABO group(32 rats).Blood samples were taken from inferior vena cava at 8,24,72 and 168 hours after operation.Plasma endotoxin level was determined with chromogenic method and plasma D-lactate level by mo-dified spectrophotometry.Results A significant el... |
| Keywords: | intestinal obstruction endotoxin D-lactate rats |
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