| 低剂量阿糖胞苷对Jurkat T淋巴细胞CD147表达的影响 |
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| 引用本文： | 王汉伟,张俊峰,魏冬,郭竹英,徐芒华,高丰厚. 低剂量阿糖胞苷对Jurkat T淋巴细胞CD147表达的影响[J]. 蚌埠医学院学报, 2011, 36(10): 1054-1057 |
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| 作者姓名： | 王汉伟 张俊峰 魏冬 郭竹英 徐芒华 高丰厚 |
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| 作者单位： | 1. 上海交通大学医学院附属第三人民医院心内科, 上海 201900;2. 上海交通大学医学院附属第三人民医院实验中心, 上海 201900 |
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| 基金项目： | 上海市教育委员会科研创新资助项目(09YZ80); 上海市宝山区科委基金资助项目(06-E-3) |
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| 摘 要： | 目的: 探讨低剂量阿糖胞苷(Ara-C)对Jurkat T淋巴细胞CD147表达的影响。方法: 实验细胞分6组,为对照组和1、5、10、20、30 ng/ml Ara-C组。采用RT-PCR、Western blot和流式细胞术分别检测Ara-C干预前后Jurkat细胞CD147基因和蛋白的表达。用倒置显微镜观察、比较Ara-C干预前后Jurkat细胞集落形成情况。结果: Ara-C各浓度组中细胞CD147 mRNA和蛋白的表达均明显高于对照组(P < 0.01),Ara-C干预组细胞集落形成明显增加。结论: 低剂量Ara-C可上调Jurkat T淋巴细胞CD147的表达,增强细胞间黏附力。
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| 关 键 词： | Jurkat细胞 阿糖胞苷 CD147 集落形成 动脉粥样硬化 |
| 收稿时间： | 2010-11-09 |
The effect of Ara-C at low concentrations on CD147 expression in Jurkat cells |
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| WANG Han-wei,ZHANG Jun-feng,WEI Dong,GUO Zhu-ying,XU Mang-hua,GAO Feng-hou. The effect of Ara-C at low concentrations on CD147 expression in Jurkat cells[J]. Journal of Bengbu Medical College, 2011, 36(10): 1054-1057 |
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| Authors: | WANG Han-wei ZHANG Jun-feng WEI Dong GUO Zhu-ying XU Mang-hua GAO Feng-hou |
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| Affiliation: | 1. Department of Cardiology, The No.3 People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201900, China;2. Center of Laboratory, The No.3 People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 201900, China |
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| Abstract: | Objective:To investigate the effect of Ara-C at low concentrations on extracellular matrix metalloproteinase inducer(CD147) expression in Jurkat cells.Methods:Jurkat cells were divided into six groups:control group and cells treated with different concentrations of Ara-C at 1,5,10,20,and 30 ng/ml.CD147 mRNA and protein expression were assayed by RT-PCR,Western blot and flow cytometry,respectively.The colony formation in Jurkat cells which was stimulated with Ara-C or not was observed by inverted microscope.... |
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| Keywords: | Jurkat cells Ara-C CD147 colony formation atherosclerosis |
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