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| 猪APOBEC3F基因克隆及真核表达载体的构建与鉴定 | |
| 引用本文： | 孙宇,罗佳,马玉媛,章金刚. 猪APOBEC3F基因克隆及真核表达载体的构建与鉴定[J]. 第三军医大学学报, 2011, 0(18): 1904-1906 |
| 作者姓名： | 孙宇 罗佳 马玉媛 章金刚 |
| 作者单位： | 1. 100850北京，军事医学科学院野战输血研究所；100017北京，解放军第305医院检验科 2. 广西大学动物科学技术学院预防兽医系,南宁,530005 3. 军事医学科学院野战输血研究所,北京,100850 |
| 基金项目： | 国家自然科学基金(3107185)~~ |
| 摘 要： | 目的 克隆猪载脂蛋白B mRNA编辑酶催化多肽样蛋白3F(apolipoprotein B mRNA editing enzyme，catalytic polypeptide 3F，APOBEC3F)基因，构建真核表达载体实现其在体外表达与鉴定。方法分离4头21周龄，体质量25～30kg的雌性健康五指山猪的外周血单个核... |
| 关 键 词： | APOBEC3F 克隆 真核表达载体 鉴定 |
Cloning of porcine apolipoprotein B mRNA editing enzyme,catalytic polypeptide-like 3F gene and construction and identification of eukaryotic expression vectors |
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| Sun Yu,Luo Jia,Ma Yuyuan,Jingang Zhang. Cloning of porcine apolipoprotein B mRNA editing enzyme,catalytic polypeptide-like 3F gene and construction and identification of eukaryotic expression vectors[J]. Acta Academiae Medicinae Militaris Tertiae, 2011, 0(18): 1904-1906 | |
| Authors: | Sun Yu Luo Jia Ma Yuyuan Jingang Zhang |
| Affiliation: | Sun Yu1,2,Luo Jia3,Ma Yuyuan1,Zhang Jingang1(1Institute of Field Transfusion,Academy of Military Medical Sciences,Beijing,100085,2 Department of Laboratory Medicine,No.305 Hospital of PLA,100017,3Faculty of Veterinary Medicine,College of Animals Sciences & Technology,Guangxi University,Nanning,Guangxi Zhuang Autonomous Regiong,530005,China) |
| Abstract: | Objective To clone the gene of porcine apolipoprotein B mRNA editing enzyme,catalytic polypeptide-like 3F(APOBEC3F)and construct the eukaryotic expression vectors for its expression and identification in vitro.Methods Peripheral blood mononuclear cells(PBMC) were isolated from blood samples of 4 female healthy Wuzhishan porcine weighing 25 to 30 kg at the age of 21 weeks.RNA was extracted from porcine PBMC using the TRAZO regent.Coding region of the APOBEC3F gene was amplified by RT-PCR.Gene fragments were ... |
| Keywords: | apolipoprotein B mRNA editing enzyme catalytic polypeptide-like 3F clone eukaryotic expression vector identification |
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