细胞因子对非肥胖性糖尿病鼠糖尿病干预治疗的实验研究

目的　研究细胞因子对非肥胖性糖尿病 (NOD)鼠糖尿病发生的影响。方法　对注射了一次大剂量的环磷酰胺 (CTX) (30 0mg/kg)后的NOD小鼠试用了T细胞增长因子 (TCGF ,1ml/d)、白细胞介素 4 (IL 4 ,5 0 0U/d) ,白细胞介素 10 (IL 10 ,5 0 0U/d)进行了干预治疗 ,并与刀豆素A (ConA ,每只鼠 5 μg/d)及载体作了对比。结果　NOD鼠经TCGF、IL 4、IL 10治疗 14d可以明显减轻注射CTX后胰岛破坏的加速 ,病理检查示胰岛炎严重程度计分分别为 4 5 (TCGF)、10 3(IL 4 )、118(IL 10 )、2 13(ConA)、2 31(载体 )。TCGF、IL 4、IL 10处理组明显轻于ConA处理组与对照组。本研究还显示经 14d的治疗 ,糖尿病发病率TCGF组为 0 / 12 ;IL 4组为 1/ 8;IL 10组为 1/ 8;对照组为 7/ 12 (P <0 0 5 )。但是对于已发病的自发性糖尿病TCGF不能使其缓解。结论　TCGF、IL 4、IL 10可减少和推迟NOD鼠糖尿病的发生

The effect of cytokines on diabetes in non-obese diabetic mice

Objective To investigate the influence of cytokines on diabetes in non obese diabetic (NOD) mouse.Methods Fifty day old NOD mice,which had been treated with CTX (300 mg/kg,one dose,i.p.),received T cell growth factor (1 ml/mouse/day in one i.p.)in TCGF group;IL 4 (500 U/d/mouse i.p.) in IL 4 group;IL 10 (500 U/day/mouse i.p.) in IL 10 group;Con A (5 μg/mouse/day in one ml,i.p.)in Con A group and vehicle (1 ml/mouse/day) in control group respectively for 14 days.Results The severity of islet pathology in TCGF,IL 4,IL 10 groups was much slighter than that in control and Con A groups (the score of severity of islet pathology was 45,103,118,213 and 231 respectively).This study also showed that CTX could accelerate destruction of pancreatic islets in almost all NOD mice (aged over 12 weeks) and induce diabetes in about half (7/12) of the same aged NOD mice within 2 weeks after injection of CTX.But no one of the mice which were treated with TCGF and only one in IL 4 and IL 10 groups after injection of CTX had diabetes in the observation period.Conclusion TCGF,IL 4,IL 10 could partly prevent diabetes in NOD mouse.