| 纳米碳酸钙对大鼠精子畸形和外周血淋巴细胞DNA的影响 |
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| 引用本文： | 宋秋坤,王慧,贺连平,张永雷,梁艳芳,王婕,田凤,仇玉兰. 纳米碳酸钙对大鼠精子畸形和外周血淋巴细胞DNA的影响[J]. 毒理学杂志, 2010, 0(2) |
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| 作者姓名： | 宋秋坤 王慧 贺连平 张永雷 梁艳芳 王婕 田凤 仇玉兰 |
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| 作者单位： | 山西医科大学毒理学教研室公共卫生学院;山西医科大学07级临床儿科; |
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| 基金项目： | 山西省高等学校科技项目(200811013); 山西医科大学博士启动基金 |
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| 摘 要： | 目的初步探索纳米碳酸钙对大鼠精子和外周血淋巴细胞DNA的影响。方法将32只雄性Wistar大鼠按体重随机分为4组,分别为阴性对照组(生理盐水),低剂量组(0.8mg/ml纳米碳酸钙),中剂量组(4mg/ml纳米碳酸钙),高剂量组(20mg/ml纳米碳酸钙)和用气管注入法对动物进行染毒,每周染毒一次,连续五周。对外周血淋巴细胞DNA的损伤用彗星实验进行分析,用精子畸形试验观察畸形率。结果染毒期间各组动物体重均有增长,各组间的增长量与对照组比较,差异均无统计学意义(P0.05);各染毒组精子畸形率均高于对照组,但是差异没有统计学意义(P0.05);各染毒组外周血淋巴细胞DNA的迁移率均明显高于阴性对照组,中、高剂量组与阴性对照组之间的差异有统计学意义(P0.05)。结论在本试验条件下,纳米碳酸钙可造成大鼠外周血淋巴细胞DNA的损伤。
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| 关 键 词： | 纳米碳酸钙 精子畸形 DNA损伤 |
The effect of nano-CaCO_3 on sperm and peripheral blood lymphocytes DNA of rats |
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| SONG Qiu-kun,WANG Hui,HE Lian-ping,ZHANG Yong-lei,LIANG Yan-fang,WANG Jie,TIAN Feng-jie,QIU Yu-lan. The effect of nano-CaCO_3 on sperm and peripheral blood lymphocytes DNA of rats[J]. Journal of Toxicology, 2010, 0(2) |
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| Authors: | SONG Qiu-kun WANG Hui HE Lian-ping ZHANG Yong-lei LIANG Yan-fang WANG Jie TIAN Feng-jie QIU Yu-lan |
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| Affiliation: | SONG Qiu-kun,WANG Hui,HE Lian-ping,ZHANG Yong-lei,LIANG Yan-fang,WANG Jie,TIAN Feng-jie,QIU Yu-lan (School of Public Health,Shanxi Medical University,Shanxi Taiyuan 030001,China) |
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| Abstract: | Objective To explore primarily the effect of nano-CaCO3 on sperm and peripheral blood lymphocytes DNA of rats. Methods 32 male rats were randomly divided into negative control group and three experimental groups which were exposed to 0. 8mg /ml,4mg /ml and 20mg /ml nano-CaCO3 ,respectively. The rats were instilled with physiological saline and different doses of nano-CaCO3 once a week for five weeks through tracheal injection method. Comet assay was performed to detect peripheral blood lymphocytes DNA damag... |
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| Keywords: | Nano-CaCO3 Sperm abnormality DNA damage |
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