高效液相色谱法测定大鼠心肌中腺苷的含量

目的：建立高效液相色谱法测定大鼠心肌组织中腺苷的含量。方法 SD大鼠随机分为心肌缺血再灌注组（ IRI）、106 Treg细胞转输＋IRI组、107Treg细胞转输＋IRI 组和假手术对照组。色谱柱为 VP-ODS C18柱（250mm ×4.6mm，5μm）；流动相为甲醇：10mmol· L-1乙酸铵（10∶90）；流速为1.0mL· min -1；柱温25℃；检测波长260nm；进样量20μL。结果腺苷的标准液与峰面积均呈良好的线性关系（r＝1.000），平均回收率分别为100.1％。日内和日间精密度分别在4.56％和4.84％以下。结论本法操作简便、准确、快速，可以用于测定大鼠心肌组织中腺苷的含量。

Determination of adenosine in myocardium of rats by HPLC

OBJECTIVE To develop a high performance liquid chromatography （ HPLC） method to determine＆amp;nbsp;the content of adenosine in myocardium of rats.METHODS Male SD rats were divided into ischemia-reperfusion group,106 Treg cell transmission treatmen ＋IRI group、107 Treg cell transmission treatment ＋IRI group and shamoper-ated group.HPLC analysis was preformed using a VP-ODS C18 column （250mm ×4.6mm,5μm）.The mobile phase consisted of methanol and 10 mmol· L-1 ammonium acetate with ratio of 10 to 90.The flow rate was 1mL· min-1 and the temperature of column was about 25℃.The detection wavelength was 260 nm and the injection volume was 20μL.RESULTS The calibration curve showed good linearity （ r=1.000 ） and the average recovery rates of aden-osine was 100.1%.The within-day and between-day precisions were less than 4.56% and 4.84%, respective-ly.CONCLUSION This analytical method is simple , rapid and specific in detecting the content of adenosine in myocardium of rats with myocardial ischemia.