| 不同时期大鼠整胚石蜡标本的制备 |
| |
| 引用本文： | 冉黎,张全波,陆长青,陈红,王凡.不同时期大鼠整胚石蜡标本的制备[J].四川解剖学杂志,2007,15(3):14-16. |
| |
| 作者姓名： | 冉黎 张全波 陆长青 陈红 王凡 |
| |
| 作者单位： | 四川大学华西基础医学与法医学院解剖教研室,成都,610041 |
| |
| 摘 要： | 目的探讨不同时期大鼠整体胚胎石蜡标本的制备。方法收集胚龄11天至19天的大鼠胚胎,经4%多聚甲醛固定、酒精梯度脱水、浸蜡、包埋技术制备石蜡标本,光镜下观察胚胎组织结构。结果不同时段的整胚石蜡标本其组织器官的脱水、透明和浸蜡均较完全,镜下观察各组织结构完整,层次清楚。结论经本实验采用的制备流程,胚胎石蜡标本效果良好。
|
| 关 键 词： | 大鼠 胚胎 包埋 |
Preparing Method of Paraffin Wax Embedding Samples of Rat Embryo at Different Developing Stages |
| |
| RAN Li,ZHANG Quan-bo,LU Chang-qing,Chen Hong,WANG Fan.Preparing Method of Paraffin Wax Embedding Samples of Rat Embryo at Different Developing Stages[J].Sichuan Journal of Anatomy,2007,15(3):14-16. |
| |
| Authors: | RAN Li ZHANG Quan-bo LU Chang-qing Chen Hong WANG Fan |
| |
| Institution: | Department of Anatomy, West China School of Preclinical and Forensic Medicine, Sichuan University, Chendu 610041 |
| |
| Abstract: | Objective To explore the preparing method of paraffin wax embedding samples of rat embryo at different developing stages.MethodsThe specimens of rat embryos at 11-19 embryonic day(E11-E19) were collected,fixed in 4% paraformaldehyde inPBS,subsequently dehydrated in series of alcohols and embedded in paraffin wax,then the tissular structure of the embryos was observed by using light microscope.ResultThrough the procedure mentioned above,The structure between different tissues were easily to be determined and no fragmentation was found in tissues under the light microscope.ConclusionThe method in this study is effective and stable,and the morphologic characters are preserved. |
| |
| Keywords: | Rat Embryo Embedding |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
