| 重组人白细胞介素15工程菌高密度发酵条件探讨 |
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| 引用本文： | 张彩,王郡甫,刘金生,许晓群,张建华,张捷,冯进波,田志刚. 重组人白细胞介素15工程菌高密度发酵条件探讨[J]. 中国生化药物杂志, 2007, 28(1): 1-3 |
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| 作者姓名： | 张彩 王郡甫 刘金生 许晓群 张建华 张捷 冯进波 田志刚 |
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| 作者单位： | 山东省医学科学院,基础医学研究所,山东省肿瘤免疫与基因工程重点实验室,山东省现代医用药物与技术重点实验室,山东,济南,250062 |
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| 基金项目： | 国家自然科学基金;山东省优秀中青年科学家科研奖励基金 |
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| 摘 要： | 目的研究表达重组人白细胞介素15(rhIL-15)工程菌高密度发酵的条件。方法采用发酵罐发酵,对影响工程菌生长和目的蛋白质表达的因素进行优化。结果以甘油为碳源可明显抑制有害物质乙酸的积累,提高工程菌的表达量和收获率。高密度发酵明显提高了目的蛋白质的表达量和工程菌的产量。结论该发酵工艺大大提高了rhIL-15工程菌的产量和表达水平,为rhIL-15的规模化生产打下了基础。
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| 关 键 词： | 基因工程菌 重组人白细胞介素15 高密度发酵 碳源 大肠杆菌 |
| 文章编号： | 1005-1678（2007）01-0001-03 |
| 收稿时间： | 2006-02-23 |
| 修稿时间： | 2006-05-11 |
Preliminary studies on high cell density fermentation of the recombinant human interluekin-15 in E.coli |
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| ZHANG Cai,WANG Jun-fu,LIU Jin-sheng,XU Xiao-qun,ZHANG Jian-hua,ZHANG Jie,FENG Jin-bo,TIAN Zhi-gang. Preliminary studies on high cell density fermentation of the recombinant human interluekin-15 in E.coli[J]. Chinese Journal of Biochemical Pharmaceutics, 2007, 28(1): 1-3 |
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| Authors: | ZHANG Cai WANG Jun-fu LIU Jin-sheng XU Xiao-qun ZHANG Jian-hua ZHANG Jie FENG Jin-bo TIAN Zhi-gang |
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| Affiliation: | Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory for Tumor Immunity and Genetic Engineering of Shandong Province, Key Laboratory for Modern Medicine and Techiology of Shandong, Province, Jinan 250062, China |
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| Abstract: | Purpose To investigate the optimal high cell density fermentation condition of recombinant human interluekin-15 in E. coli. Methods The effects of the pH range, air input, incubation time and fedbatch carbon sources on the expression level of rhIL-15 and cell output were analysed. Results With the glycerol as the carbon sources it inhibited the accumulation of acetic acid and increased the yield of E. coli and the expression level of rhIL-15. High cell density fermentation enhanced the output of E. coli and the expression level of rhIL-15 significantly. Conclusion The optimized fermentation condition for recombinant E. coli raised significantly the high expression level of rhIL-15. It is suitable for the fermentation of rhIL-15 on a large scale. |
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| Keywords: | genetic engineering bacteria recombinant human interluekin-15 higkcell density fermentation carbon sources E. coli |
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