氯化甲基汞和甲氨蝶呤对Jurkat细胞凋亡的影响

目的通过体外细胞实验研究氯化甲基汞(Methyl mercury chloride,MMC)和甲氨蝶呤(Methotrexate,MTX)对人T淋巴细胞白血病细胞(Jurkat)凋亡的影响。方法 Jurkat细胞经1.25、2.5、5、10、20μmol.L-1 MMC和MTX作用24h。应用四甲基偶氮唑盐(MTT)比色法检测不同浓度MMC和MTX对Jurktat细胞增殖的抑制作用;采用流式细胞术应用AnnexinⅤ-FITC/PI双染法检测不同浓度MMC和MTX对Jurktat细胞凋亡的影响。结果 MMC和MTX作用Jurkat细胞24h,均能够抑制Jurkat细胞增殖,促进其凋亡;且与药物浓度正相关。10μmol.L-1 MMC的细胞凋亡率为(54.65±3.15)%,与对照组(0.15±0.03)%以及MTX组(26.32±2.64)%相比差异均有统计学意义(P<0.05)。结论 MMC和MTX均能够抑制Jurkat细胞增殖,诱导其凋亡;但前者作用更为明显。

Effect of Methylmercury and Methotrexate on Apootosis of Jurkat cells

Objective To study the effect of methyl mercury chloride（MMC） and methotrexate（MTX） on apoptosis of T lymphocytes leukemia cells（Jurkat） in vitro.Methods MMC and MTX treated Jurkat cells in 1.25,2.5,5,10 and 20 μmol·L-1 for 24h.MTT assay was performed to evaluate the proliferation inhibitory effect of MMC and MTX with different concentrations on cultured Jurkat cells.Cell apoptosis rates were detected by the way of AnnexinⅤ-FITC/PI.Results When Jurkat cells were treated with MMC and MTX for 24 h,both MMC and MTX could inhibit the proliferation of Jurkat cells in vitro;and the inhibit rates were significantly higher than those in MTX group（P〈0.05）.The inhibitory effect was in a dose-dependent manner.The apoptosis rate of cells treated with 10 μmol·L-1 MMC for 24 h was（54.65 ±3.15）%.Compared with control group（0.15±0.03）% and MTX group（26.32±2.64）%,there was significant difference（P〈0.05）.Conclusion MMC and MTX could induce apoptosis of Jurkat cells.Effect of MMC was more significant.