| 邻苯二甲酸单苄酯单克隆抗体的制备 |
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| 引用本文： | 李乐,任洪林,李岩松,周玉,冯晓丽,刘艳艳,唐峰,李兆辉,王光明,卢士英,柳增善. 邻苯二甲酸单苄酯单克隆抗体的制备[J]. 中国实验诊断学, 2012, 16(3): 417-419 |
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| 作者姓名： | 李乐 任洪林 李岩松 周玉 冯晓丽 刘艳艳 唐峰 李兆辉 王光明 卢士英 柳增善 |
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| 作者单位： | 吉林大学,人兽共患病研究所教育部重点实验室,畜牧兽医学院,吉林,长春130062 |
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| 基金项目： | 国家自然科学基金项目（31071539）; 吉林大学农学部引进人才科研启动项目（4305050102A8） |
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| 摘 要： | 目的制备抗邻苯二甲酸单苄酯(mono-benzyl phthalate,MBzP)的单克隆抗体。方法本实验利用活泼酯法,将邻苯二甲酸单苄酯分别与载体蛋白BSA和OVA偶联,获得完全抗原MBzP-BSA和MBzP-OVA,用MBzP-BSA免疫Balb/c小鼠,制备杂交瘤细胞,利用ELISA方法检测阳性杂交瘤细胞株分泌抗体的效价。结果获得了稳定分泌抗MBzP的细胞株1B9,以该细胞株体内诱生腹水制备腹水型单克隆抗体,纯化后效价为1∶128 000。结论成功制备了抗邻苯二甲酸单苄酯的单克隆抗体。
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| 关 键 词： | 邻苯二甲酸单苄酯 单克隆抗体 腹水效价 |
The Perparation of Monoclonal Antibody Against MBzP |
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| Affiliation: | LI Le,REN Hong-lin,LI Yan-song,et al.(Key Laboratory for Zoonosis Research,Ministry of Education,Institute of Zoonosis,College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China) |
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| Abstract: | Objective To prepare monoclonal antibody against MBzP（mono-benzyl phthalate）.Methods MBzP was linked with vector protein BSA and OVA to produce the completed antigen（MBzP-BSA and MBzP-OVA）.Balb/c mice were immunized with MBzP-BSA to make hybridoma cells.Use ELISA to detect the titer of antibody which secreted by the hybridoma cells.Results The cell line 1B9 which can steadily produce monoclonal antibody against MBzP was obtained.The titer of the purified ascite was 1∶128 0000.Conclusion Successfully obtained the monoclonal antibody against MBzP. |
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| Keywords: | MBzP monoclonal antibody ascite |
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