Klotho基因转染促进脂肪间充质干细胞增殖的实验研究

目的探讨klotho基因转染对脂肪间充质干细胞(ADSCs)增殖的影响。方法将含有klotho全长基因的真核质粒转染至从脂肪组织分离的ADSCs中,采用CCK-8法测定转染后细胞增殖率,western blot法检测转染后细胞周期蛋白D1的表达变化,流式细胞术检测转染前后ADSCs的细胞周期变化。结果转染klotho基因96h后AD-SCs的细胞增殖率为170.50%;流式细胞术检测结果显示,ADSCs转染klotho基因S期和G2期细胞明显增多;west-ern blot分析显示ADSCs转染klotho基因组细胞周期蛋白D1增高。结论转染klotho基因有促进ADSCs增殖的作用。

Experimental study on proliferation of ADSCs induced by klotho gene transfection

Objective To investigate proliferation of ADSCs induced by klotho gene transfection.Methods ADSCs were isolated from liposuction specimens and transfected with the klotho gene expression plasmid.The proliferation rate of ADSCs was detected by CCK-8,the expression of cyclinD and the cell cycle were detected by western blot and flow cytometry.Results When ADSCs were transfected with the vector containing klotho gene,the proliferation rate of ADSCs was 170.50% after 96h of transfection.Flow cytometric analysis showed that the number of cells in S and G2 phase had increased significantly.Western blot analysis indicated that cyclinD1 expression in klotho gene transfection group had also increased.Conclusion klotho gene can promote the proliferation of the ADSCs.