皮肤鳞癌组织中p14ARF、E-cad、FOS和JUN基因甲基化状态的分析

目的通过检测皮肤鳞癌及正常皮肤组织中p14ARF、E-cad、FOS、JUN基因启动子区域CpG岛的甲基化状态,探讨p14ARF、E-cad、FOS、JUN在皮肤鳞癌发生过程中的作用及诊断意义。方法采用甲基化特异性PCR(MSP)技术,检测30例皮肤鳞癌组织和20例正常皮肤组织基因启动子区附近CpG岛的甲基化情况。结果 30例皮肤鳞癌组织中发现p14ARF、E-cad基因高甲基化例数分别为18例(60.0%)和11例(37.6%),而20例正常组织分别为1例(5.0%)和2例(10.0%);30例皮肤鳞癌FOS、JUN基因非甲基化分别为22例(73.3%)和25例(83.3%);20例正常组织非甲基化分别为6例(30.0%)和1例(5%)。采用χ2检验进行统计分析,存在统计学差异(P<0.05)。结论 p14ARF、E-cad、FOS、JUN基因启动子区域异常甲基化状态与皮肤鳞癌发病联系紧密。

Analysis of methylation status of p14ARF,E-cad,FOS and JUN in skin squamous cell carcinoma

Objective To investigate the abnormal promoter methylation status of p14ARF、E-cad、FOS、JUN in skin squamous cell carcinoma.To analyze the value of these genes in the diagnosis of skin squamous cell carcinoma.Methods Promotor methylation of p14ARF、E-cad、FOS and JUN in 30 cases of skin squamous cell carcinoma,and 20 cases of normal skin tissues were detected by methylation-specific PCR.Results The hypermethylation rate of p14ARF、E-cad were 60.0 % and 37.6 % respectively in cancer specimen;while the methylation rate of p14ARF、E-cad were 5.0% and 10.0% respectively in the normal tissue;The hypomethylation rate of FOS、JUN were 73.3% and 83.3% respectively;while the hypomethylation rate of FOS、JUN in normal group were just 30.0% and 5.0% respectively.Significant difference between mormal and tumor group.Conclusion The promotor methylation of p14ARF、E-cad、FOS、JUN was related closely to the progression of skin squamous cell carcinoma,the methylation status of p14ARF、E-cad、FOS and JUN may be applied as prognosis markers in skin squamous cell carcinoma.