Silk fibroin nanoparticles for enhanced bio-macromolecule delivery to the retina |
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Authors: | Pianpian Yang Yixuan Dong Di Huang Hu Liu Xin Pan |
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Institution: | 1. School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou, China;2. School of Pharmacy, Memorial University of Newfoundland, St. John's, NF, Canada |
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Abstract: | The aim of this study was to investigate intravitreal injection of silk fibroin nanoparticles (SFNs) encapsulating bio-macromolecules, achieving enhanced drug bioavailability, and extended retention in retina. SFNs were prepared with regenerated silk fibroin using desolvation method with fluorescein isothiocyanate labeled bovine serum albumin (FITC-BSA) as bio-macromolecular model drug encapsulated. In vitro physicochemical properties and in vitro drug release of FITC-BSA loaded SFNs (FITC-BSA-SFNs) were evaluated. Cytotoxicity, cellular uptake, and retention of FITC-BSA-SFNs were determined in human retinal pigment epithelial cell line (ARPE-19). In addition, in vivo distribution and safety of intravitreally administered FITC-BSA-SFNs were investigated in New Zealand white rabbits. The particle size of FITC-BSA-SFNs was 179.1?±?3.7?nm with polydispersity index of 0.102?±?0.033 and the zeta potential was greater than ?25?mV. FITC-BSA-SFNs exhibited excellent biocompatibility with no cytotoxicity observed within 24 and 48?h in AREP-19 cells. Compared to FITC-BSA solution, FITC-BSA-SFNs showed enhanced cellular uptake and prolonged retention. Furthermore, FITC-BSA-SFNs achieved accumulated distribution and extended retention in retina in vivo following intravitreal injection compared to a single administration of free drug solution. Therefore, this bio-macromolecule delivery platform based on SFNs could have great potential in the treatment of posterior segment disorders. |
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Keywords: | Silk fibroin nanoparticles bio-macromolecules ocular drug delivery posterior segment diseases intravitreal injection |
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