焦炉工人血浆BPDE-白蛋白加合物与外周血淋巴细胞DNA损伤水平的相关关系

目的探讨焦炉工人早期生物效应标志物DNA损伤与生物有效剂量标志物苯并(a)芘二氢二醇环氧化物(BPDE)-白蛋白加合物之间的关系。方法选取某焦化厂207名焦炉工人作为接触组,102名非焦炉作业人员为对照组,采用反相高效液相色谱法测定血浆中BPDE-白蛋白加合物浓度,采用彗星实验测定外周血淋巴细胞DNA损伤水平。结果焦炉工人的血浆BPDE-白蛋白加合物浓度和外周血DNA损伤水平明显高于对照组(P<0.01)。使用多元logistic回归分析校正了个体的工龄、吸烟和饮酒状况后,焦炉工人BPDE-白蛋白加合物浓度增高的危险度是对照组的1.72倍(P<0.05),焦炉工人DNA损伤水平增高的危险度是对照组的1.96倍(P<0.05)。BPDE-白蛋白加合物与DNA损伤水平在焦炉工人中存在明显的正相关关系(Spearman偏相关系数=0.235,P<0.01),但在对照组中不存在相关关系(Spearman偏相关系数=0.093,P>0.05)。结论焦炉工人生物有效剂量标志物BPDE-白蛋白加合物和早期生物效应标志物DNA损伤之间存在明显相关。

Relationship between Plasma BPDE-Albumin Adduct and DNA Damage of Peripheral Blood Lymphocytes among Coke Oven Workers

Objective To explore the relationship between the DNA damage of biomarkers for early biological effects in coke oven workers and BPDE-Alb adducts.Methods 207 male coke oven workers were selected as the exposed group and 102 unexposed to coke oven as the controls.Reverse-phase high-performance liquid chromatography was used to measure BPDE-Alb adduct concentrations in plasma and alkaline comet assay for DNA damage in peripheral blood lymphocytes.Results The concentration of BPDE-Alb adducts and the Olive tail moment(Olive TM)in exposed group were significantly higher than those in control group(P<0.05).Multivariate logistic regression analysis revealed that the odds ratio(OR)for BPDE-Alb adduct and Olive TM associated with the exposure were 1.72 and 1.96 respectively.Results showed that there was significant correlation between the concentrations of BPDE-Alb adduct and Olive TM levels in exposed group(rs=0.235,P<0.01)but not in the control group(rs=0.093,P>0.05).Conclusions The relationship between biomarkers of biological effective and early biological effects was significant among coke oven workers.