胶体金免疫层析试纸条对云南鼠疫现场材料的检测

目的评价检测鼠疫抗原及抗体胶体金免疫层析试纸条(G ICA及RG ICA)对现场材料的检测效果。方法采用G ICA分别对采自云南省的607份血清标本(查鼠疫抗体)及572份鼠脏器标本(查鼠疫抗原)进行检测,同时以血凝试验(IHA及R IHA)与酶联试验(ELISA及F1-ELISA)作为对照。结果(1)在对607份血清标本的检测中,G ICA、IHA及ELISA三种方法的符合率中度,而G ICA的敏感性比IHA与ELISA分别高111%和90%;(2)在对572份鼠脏器标本的检测中,RG ICA、R IHA及F1-ELISA三种方法的符合率中度,而RG ICA的敏感性比R IHA与F1-ELISA分别高100%和14.3%。结论检测鼠疫的G ICA及RG ICA特异性强、灵敏度高、简便快速,有较大的推广应用价值。

Testing Yunnan Field Samples of Yersinia pestis with Gold Immunochromatographic Assay

Objective To evaluate the effectiveness of testing field samples of Yersinia pestis with gold immunochromatographic assay(GICA) and reverse GICA(RGICA).Methods We tested 607 serum samples and 572 mice viscera samples with GICA and RGICA,at the same time with IHA(RIHA) and ELISA(F1-ELISA) as control.Results(1)There were moderate consistence among GICA,IHA and ELISA in testing 607 serum samples.Moreover,GICA was more sensitive than IHA and ELISA(111% and 90% respectively).(2) There are moderate consistence among RGICA,RIHA and F1-ELISA in testing 572 viscera samples.Moreover,RGICA was more sensitive than RIHA and F1-ELISA(100% and 14.3% respectively).Conclusions GICA and RGICA are sensitive,specific,simple and rapid assays for detecting Yersinia pestis.GICA and RGICA have good prospect in field testing of plague.