柴胡皂苷对芍药苷在Caco-2细胞模型中 吸收转运的影响

目的:研究柴胡皂苷a,d对芍药苷在Caco-2细胞模型中转运的影响。方法:用超高压液相法测定药物浓度,计算表观渗透系数,比较不同浓度芍药苷溶液、及芍药苷配伍柴胡皂苷a,d后在Caco-2细胞模型中双向转运的差别;电阻仪测定电阻,比较试验前后细胞电阻的差异。结果:芍药苷通过Caco-2细胞单层的转运量4 h内随时间延长呈线性增大,其吸收渗透系数较小,浓度为20,50,100μmol.L-1时,吸收渗透系数分别为(0.98±0.10)×10-6,(0.92±0.09)×10-6,(0.89±0.04)×10-6cm.s-1;芍药苷与柴胡皂苷a,d配伍后,吸收渗透系数与单体比较分别增加了2.37,2.54倍,且试验后细胞电阻有显著降低。结论:柴胡皂苷a,d能促进芍药苷的肠道吸收,可能与柴胡皂苷能打开Caco-2细胞间的紧密连接有关。

Impact of saikosaponin on absorption and transport of paeoniflorin in Caco-2 cell model

Objective: To study the impact of saikosaponin on absorption and transport of paeoniflorin in Caco-2 cell model.Method: The concentration of paeoniflorin in cell culture medium was measured by UPLC and the apparent permeability coefficients(P app) was calculated to study differences in bi-direction transport of paeoniflorin solutions of different concentrations and its compatibility with saikosaponin a,and saikosaponin d in Caco-2 cell model.Meanwhile,the electric resistance of Caco-2 cell was determined before and after the experiment.Result: The amount of paeoniflorin increased linearly with the transport of Caco-2 cell monolayer in 4 h,with a lower absorptive permeability,which was(0.98 ± 0.10) × 10-6,(0.92 ±0.09) ×10-6,(0.89 ±0.04) ×10-6 cm.s-1 at the concentration of 20,50,100 μmol.L-1,respectively.After compatibility with saikosaponin a and saikosaponin d,the absorptive permeability of paeoniflorin increased by 2.37 times and 2.54 times,respectively,while the electric resistance of Caco-2 cell was decreased significantly after the experiment.Conclusion: Saikosaponin a,d can enhance the absorption of paeoniflorin in Caco-2 cell monolayer,which may be related to saikosaponin’s ability to open up intercellular tight junctions among Caco-2 cells.