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| 黄连中胆碱的分离及其对小檗碱在HepG2 细胞中糖代谢作用的影响 | |
| 引用本文： | 陈红英,李学刚,叶小利,晋亚楠,黄静,吴金凤.黄连中胆碱的分离及其对小檗碱在HepG2 细胞中糖代谢作用的影响[J].中国中药杂志,2012,37(12):1771-1774. |
| 作者姓名： | 陈红英 李学刚 叶小利 晋亚楠 黄静 吴金凤 |
| 作者单位： | 1. 西南大学药学院,重庆400715;西南科技大学生命科学与工程学院,四川绵阳621010 2. 西南大学药学院,重庆,400715 3. 西南大学生命学院,重庆,400715 |
| 基金项目： | 国家"重大新药创制"科技重大专项(2010ZX09401-306-3-10);科技部"十二五"科技支撑计划项目(2011BAI13B02-1);重庆市科委重大专项项目(CSTC2008AA5021);重庆市科委攻关项目(CSTC 2010AC5007) |
| 摘 要： | 目的:分离黄连亲水性成分,研究其对小檗碱在HepG2细胞中糖代谢作用的影响。方法:黄连亲水性部位高速逆流色谱制备后,硅胶柱色谱法进行分离。MTT法检测HepG2细胞活力,试剂盒检测细胞消耗的葡萄糖。结果:首次从黄连水溶性组分中分离出胆碱。在实验浓度范围内,胆碱能显著增强小檗碱促进细胞吸收葡萄糖的能力,并显著改善小檗碱对细胞生长抑制的影响。与同剂量的小檗碱相比,100 mg.L-1胆碱与38 mg.L-1小檗碱混合后,细胞对葡萄糖的消耗增加34%,存活率上升到75%。结论:胆碱在增强小檗碱促进细胞吸收葡萄糖和减小小檗碱对细胞的毒副作用方面存在显著协同作用。 |
| 关 键 词： | 黄连 小檗碱 胆碱 降糖 HepG2细胞 |
| 收稿时间： | 2011/11/4 0:00:00 |
Separation of choline from Coptidis Rhizoma and impact on glucose metabolism of berberine in HepG2 cells |
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| CHEN Hongying,LI Xuegang,YE Xiaoli,JIN Ya''nan,HUANG Jing and WU Jinfeng.Separation of choline from Coptidis Rhizoma and impact on glucose metabolism of berberine in HepG2 cells[J].China Journal of Chinese Materia Medica,2012,37(12):1771-1774. | |
| Authors: | CHEN Hongying LI Xuegang YE Xiaoli JIN Ya'nan HUANG Jing and WU Jinfeng |
| Institution: | School of Pharmaceutical Science, Southwest University, Chongqing 400715, China;School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China;School of Pharmaceutical Science, Southwest University, Chongqing 400715, China;School of Life Science, Southwest University, Chongqing 400715, China;School of Pharmaceutical Science, Southwest University, Chongqing 400715, China;School of Pharmaceutical Science, Southwest University, Chongqing 400715, China;School of Pharmaceutical Science, Southwest University, Chongqing 400715, China |
| Abstract: | Objective: To investigate action of hydrophilic constituents from Coptidis Rhizoma on glucose-lowering effect metabolism of berberine in HepG2 cells. Method: Hydrophilic fractions of Coptidis Rhizoma were prepared by high speed counter current chromatography and separated by silica gel column chromatography. MTT assay was used to monitor the proliferation of HepG2 cells,and Kit was used to test the glucose consumption in culture solution. Result: Choline was separated from Coptidis Rhizoma for the first time. Cell assay showed choline can significantly increase the glucose lowering effect of berberine and improve the cytotoxicity of berberine within test concentration. Compared with same dose of berberine,berberine 38 mg·L-1in combination with choline 100 mg·L-1 can make glucose consumption increase by 34% and elevate cell livability up to 75% in HepG2 cells. Conclusion: The results suggest that choline had a synergistic effect on improving glucose absorption of berberine and decreasing cytotoxicity of berberine. |
| Keywords: | Coptidis Rhizoma berberine choline glucose lowering HepG2 cells |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |