| 正交试验优选人参多糖除蛋白工艺 |
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| 引用本文： | 宋利华,萧 伟.正交试验优选人参多糖除蛋白工艺[J].世界科学技术-中医药现代化,2012,14(2):1502-1507. |
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| 作者姓名： | 宋利华 萧 伟 |
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| 作者单位： | 南京中医药大学药学院/江苏康缘药业股份有限公司 连云港 222001;江苏康缘药业股份有限公司/中药制药过程新技术国家重点实验室 连云港 222001 |
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| 基金项目： | 科学技术部国家“973计划”项目（2010CB735604）：新药研制过程化学机理，负责人：萧伟。 |
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| 摘 要： | 目的:优选人参多糖的最佳除蛋白工艺。方法:以蛋白清除率、多糖保留率和糖醛酸保留率为指标,比较了两种不同除蛋白方法(Sevag法和TCA法)的效果,并采用正交试验优选最佳除蛋白工艺。结果:Sevag法的除蛋白效果优于TCA法,人参多糖的最佳除蛋白工艺为5%人参多糖溶液中加入1/3倍体积的Sevag试剂,V氯仿∶V正丁醇为4∶1,共除6次。结论:该工艺除蛋白效率较高,可用于人参多糖的分离精制。
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| 关 键 词： | 人参多糖 除蛋白 正交试验 |
| 收稿时间： | 2011/11/3 0:00:00 |
| 修稿时间： | 2012/1/19 0:00:00 |
Optimization on Protein-removing Technology of Ginseng Polysaccharides by Orthogonal Test |
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| Song Lihua and Xiao Wei.Optimization on Protein-removing Technology of Ginseng Polysaccharides by Orthogonal Test[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2012,14(2):1502-1507. |
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| Authors: | Song Lihua and Xiao Wei |
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| Institution: | Nanjing University of Traditional Chinese Medicine, Nan Jing 210000, China; Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China;Jiangsu Kanion Pharmaceutical Co., Ltd., Lianyungang 222001, China; State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process, Lianyungang 222001, China |
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| Abstract: | This study was aimed to optimize conditions for protein-removing from ginseng polysaccharides.The protein-removing rate,retaining rate of polysaccharides and uronic acid were used as indicators.Results of two protein-removing methods(i.e.,Sevag method and TCA method) were compared.And the optimized protein-removing conditions were obtained by orthogonal test.The results of Sevag method were better than TCA method.The optimized condition for protein-removing from ginseng polysaccharides was that Sevag reagent volume was equal to 1/3 times of 5% ginseng polysaccharides solution.And the volume ratio of CHCl3 and normal butanol was 4∶1.The protein-removing process was conducted 6 times.It was concluded that the method is efficient and can be used for the separation and refinement of ginseng polysaccharides. |
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| Keywords: | Ginseng polysaccharides protein-removing orthogonal test |
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