白术内酯I对免疫性肝损伤的保护作用

目的:研究白术内酯Ⅰ对卡介苗(BCG)联合脂多糖(LPS)诱导的免疫性肝损伤的保护作用,并探讨其可能的机制。方法:昆明种雄性小鼠随机分为正常对照组、模型组、阳性对照药联苯双酯组和白术内酯Ⅰ低、中、高剂量组(60,120,240mg.kg-1),每组12只。BCG联合LPS诱导免疫性肝损伤,比较各组小鼠的肝脏、脾脏系数,检测小鼠血清丙氨酸氨基转移酶(ALT)和门冬酸氨基转移酶(AST)的含量、肝匀浆液中丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-px)的含量以及血浆和肝组织中肿瘤坏死因子α(TNF-α),NO,诱导型一氧化氮合酶(iNOS)含量,HE染色切片观察肝组织病理学变化。结果:白术内酯Ⅰ与联苯双酯均可显著降低免疫性肝损伤小鼠增加的肝脏指数、脾脏指数(P<0.05),改善肝脏组织病理学变化和肝脏组织病理学分级,减轻BCG联合LPS所致肝损伤的炎症反应;对免疫性肝损伤中肝匀浆MDA产生和GSH-px水平有明显改善作用。结论:白术内酯I对免疫性肝损伤具有显著的保护作用,并且在实验剂量范围内呈显著的剂量依赖性。抑制NO,iNOS,TNF-α等炎症介质的释放或过强表达可能是其主要的作用机制。

Protective effect of atractylenolide I on immunological liver injury

Objective: To study the protective effect of atractylenolide Ⅰ on immunological liver injury induced by BCG and LPS.Method: Kunming mice were randomly divided into 6 groups: the normal group,the model group,positive control biphenyl group,the atractylenolide I high does group,the atractylenolide Ⅰ middle dose group and the atractylenolide Ⅰ low dose group(60,120,240 mg·kg-1),with 12 mice in each group.Immunological liver injury in mice was induced by BCG and LPS to compared liver index and spleen index and detect content of serum ALT,AST,MDA and GSH-px in serum and NO,iNOS,TNF-α in serum and liver homogenate.Liver pathological changes were observed by HE staining.Result: Both of atractylenolide Ⅰ and biphenyl remarkably decrease the increased live index and spleen index(P<0.05),improve the histopathological changes in liver and pathological grades of liver tissues and relieve the inflammatory reaction induced by BCG and LPS.They showed a notable effect in improving MDA and GSH-px in serum.Conclusion: Atractylenolide Ⅰ can obviously protect immunological injury liver a dose-dependent manner within the range of test doses.Its mechanism may be related to release or over expression of inhibitory inflammatory medium such as NO,iNOS and TNF-α.