具抗氧化活性的中药有效组分的配伍研究

目的:以4种中药有效组分配伍的抗氧化活性为研究对象,探索适合中药多组分多指标"组效关系"的研究方法。方法:基于LARS回归算法和综合权重分析方法,按照均匀试验设计-药效试验-数学建模(模型验证)-综合药效评价程序,进行甘草总黄酮、银杏叶提取物、淫羊藿总黄酮和黄芪总苷4个中药组分不同配伍剂量体外清除DPPH和多环芳烃的研究。结果:采用改进最小角回归分析和综合权重法,评价清除DPPH和多环芳烃的最优综合药效,中药组分配伍最佳剂量组合为甘草总黄酮-银杏叶提取物-淫羊藿总黄酮-黄芪总苷(1∶0.254 5∶0.007 6∶0.011 5)。结论:上述中药组分配伍能有效清除多环芳烃和DPPH,具有良好的抗氧化作用。本实验采用的均匀设计结合综合权重法,适合"非线性、小样本"生物实验数据分析,是进行中药组分配伍筛选评价的适合研究方法。

Study on compatibility of traditional Chinese medicine active constituents with antioxidant activity

Objective: To find a research method suitable to multi-component multi-index composition-activity relationship of traditional Chinese medicines(TCMs) with the antioxidant activity of four compatible active constituents of TCMs as the subject.Method: LARS-based regression algorithm and comprehensive weight coefficient method were adopted to study in vitro clearance of DPPH and polyaromatic hydrocarbon with different doses of four compatible active constituents——total flavonoids of Glycyrrhizae Radix et Rhizoma(TFG),Ginkgo Folium extract(GBE),total flavonoids of Epimedii Folium(TFE) and astragaloside(AST) according to the comprehensive efficacy assessment procedures,that is test design,efficacy test and mathematical modeling(model verification).Result: LARS and comprehensive weight coefficient method was adopted to assess the optimal comprehensive efficacy of clearing DPPH and polyaromatic hydrocarbon.The results showed that the optimal dose combination of compatible constituents is TFG-GBE-TFE-AST 1∶0.254 5∶0.007 6∶0.011 5.Conclusion: The above compatibility of TCMs constituents can effectively clear polyaromatic hydrocarbon and DPPH with a good antioxidation.The uniform design adopted in this experiment,in combination of synthetic weight method,is suitable to the data analysis of "non-linear and small-sample" biotic experiment and the study on screening and assessment of compatibility of TCMs constituents.