大叶茜草素及羟基茜草素在大鼠体肠吸收研究

目的:研究大叶茜草素及羟基茜草素在大鼠各肠段中的吸收动力学特征。方法:采用大鼠在体单向肠灌流模型对大叶茜草素和羟基茜草素在大鼠各肠段的吸收特性进行研究。用酚红标记法校正循环液体积。结果:不同质量浓度的大叶茜草素(12.33,24.66,49.32 mg.L-1)及羟基茜草素(8.455,16.91,33.82 mg.L-1)在各肠段的吸收量顺浓度梯度,渗透系数呈上升趋势且均大于0.2×10-4cm.s-1,在相同浓度下大叶茜草素及羟基茜草素在各肠段Peff表现为相同趋势,依次为结肠>十二指肠>回肠>空肠,且有显著性差异(P<0.05)。结论:大叶茜草素及羟基茜草素在大鼠肠道内为高渗透性药物,各肠段均有吸收,且在结肠部位可能存在特异性吸收。

Study on intestinal absorption of mollugin and purpurin in rats

Objective: To study the absorption kinetic characteristics of mollugin and purpurin in each intestinal segment of rats. Method: The in situ single-way perfusion rat model was established to study absorption characteristics of mollugin and purpurin in each intestinal segment of rats. The volume of recirculation fluid was regulated by phenol red. Result: Different quality concentrations(12.33, 24.66, 49.32 mg·L^-1) of mollugin and(8.455, 16.91, 33.82 mg·L^-1) purpurin showed a concentration gradient of absorption dose in each intestinal segment, with the osmotic coefficient increasing to more than 0.2×10^-4 cm·s^-1. In the same concentration, mollugin and purpurin showed an identical trend of P_eff in each intestinal segment in the order of colon>duodenum>ileum>jejunum, with a significant difference(P<0.05). Conclusion: Mollugin and purpurin are highly permeable in rat intestinal segments, with absorption in each segment, while the specific absorption existed in the colon segment.