Inhibitory effect of oxymatrine on serum hepatitis B virus DNA in HBV transgenic mice

AIM:To study the inhibitory effect of oxymatrine on serumhepatitis B virus (HBV) DNA in HBV transgenic mice.METHODS:HBV transgenic mice model was establishedby microinjection,and identified by HBV DNA integrationand replication.Transgenic mice with replicating HBV weredivided into 3 groups,and injected with normal saline(group A,n=9),50 mglkg (group B,n=8) and 100 mg/kg(group C,n=9) oxymatrine intraperitoneally once a dayfor 30d,respectively.Quantitation of serum HBV DNA inHBV transgenic mice was performed by competitivepolymerase chain reaction (PCR) in combination with DNAhybridization quantitative detection technique before andafter treatment.RESULTS:Compared with pre-treatment,the serum HBVDNA in group A (F=1.04,P=0.9612) and group B (F=1.13,P=0.8739) had no changes after treatment.However,in groupC serum HBV DNA was significantly decreased (F=13.97,P=0.0012).The serum HBV DNA after treatment was lowerin group C than in groups B and A (F=8.65,P=0.0068;F=12.35,P=0.0018;respectively).The serum HBV DNAafter treatment was lower in group B than in group A,butthere was no statistical significance (F=1.43,P=0.652).CONCLUSION:Oxymatrine has inhibitory effects on serumHBV DNA in HBV transgenic mice.