首页 | 本学科首页   官方微博 | 高级检索  
     

枸杞多糖对慢性辐射小鼠细胞凋亡及bc1-2基因表达的影响
引用本文:李德远,汤坚,徐现波,苏喜生,钟飞,钟进义. 枸杞多糖对慢性辐射小鼠细胞凋亡及bc1-2基因表达的影响[J]. 营养学报, 2005, 0(3)
作者姓名:李德远  汤坚  徐现波  苏喜生  钟飞  钟进义
作者单位:江南大学食品学院,江南大学食品学院,军事经济学院营养食品所,军事经济学院营养食品所,军事经济学院营养食品所,青岛大学医学院 无锡214036 军事经济学院营养食品所,武汉430035,无锡214036,武汉430035,武汉430035,武汉430035,266012
基金项目:总后勤部军需部(No.需9802)
摘    要:目的:探讨枸杞多糖(LBP)对辐射小鼠细胞凋亡及凋亡相关基因bcl-2表达的影响。方法:以水提取-乙醇沉淀法制备枸杞多糖,并制成0.8%的饲料,给予受试小鼠(枸杞多糖组)。正常对照组、辐射对照组给予普通饲料。除正常对照组外,另两组均用60Coγ射线对动物进行全身性照射,每天照射1次,每天照射剂量为0.084Gy,每周照射5d,连续照射6w,照射总剂量为2.52Gy。检测骨髓微核率、睾丸精母细胞染色体畸变、精子畸形率、肝细胞caspase-3mRNA表达水平、细胞凋亡及凋亡相关基因bc1-2表达等指标。结果:LBP可使辐射引起的微核率、染色体畸变、及精子畸形率显著降低,骨髓细胞增殖活性提高,凋亡率降低,使辐射小鼠bc1-2基因表达提高、caspase-3mRNA表达水平降低。结论:枸杞多糖的抗辐射作用与其调控细胞bc1-2基因表达,影响细胞凋亡有关。

关 键 词:枸杞多糖  细胞凋亡  bcl-2  基因表达

EFFECTS OF POLYSACCHARIDE FROM LYCIUM BARBARUM ON CELL APOPTOSIS AND bc1-2 GENE EXPRESSION IN IRRADIATED MICE
LI De-yuan,,TANG Jian,XU Xian-bo,SU Xi-sheng,ZHONG Fei,ZHONG Jin-yi. EFFECTS OF POLYSACCHARIDE FROM LYCIUM BARBARUM ON CELL APOPTOSIS AND bc1-2 GENE EXPRESSION IN IRRADIATED MICE[J]. Acta Nutrimenta Sinica, 2005, 0(3)
Authors:LI De-yuan    TANG Jian  XU Xian-bo  SU Xi-sheng  ZHONG Fei  ZHONG Jin-yi
Affiliation:LI De-yuan1,2,TANG Jian1,XU Xian-bo2,SU Xi-sheng2,ZHONG Fei2,ZHONG Jin-yi3
Abstract:Objective:To study the effects of polysaccharide from Lycium barbarum (LBP) on cell apoptosis and bc1-2 gene expression in irradiated mice. Methods:Forty eight Kunming mice were divided into three groups (LBP group, radiation group and normal group). LBP chow was prepared through adding 0.8%LBP to normal chow and was supplied to LBP group. Normal chow was supplied to normal group and radiation group. LBP group and radiation group were exposed to whole-body 60Co γ-rays at the dose of 0.084 Gy/day for 6 w, five times a week and the total dose was 2.52Gy. Then the micronucleus frequency of polychromatic erythrocytes(MF),chromosome and sperm aberration frequency, caspase-3 mRNA expression, cell apoptosis and bcl-2 gene expression were detected. Results:LBP could significantly lower MF, chromosome and sperm aberration frequency, and cell apoptosis , and it could increase the proliferation activity of bone marrow cells and bc1-2 gene expression in irradiated mice and decrease caspase-3 mRNA expression.Conclusion:The radioprotective effect of LBP is related to regulation of cell apoptosis and bc1-2 gene expression.
Keywords:Lycium barbarum polysaccharide(LBP)  cell apoptosis  bcl-2  gene expression
本文献已被 CNKI 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号