HPLC研究玉屏风散活性组分群一釜法提取效果

目的：研究玉屏风散中活性成分群(黄芪甲苷、升麻苷及5-O-甲基维斯阿米醇苷)的提取条件。方法：采用一釜法提取玉屏风散中活性成分群,HPLC分析各活性成分含量。以提取物中活性成分含量及得率为指标,通过正交实验考察乙醇体积分数、溶剂用量、提取时间和提取温度4个因素对提取效果的影响。结果：较佳的提取条件为：单位质量(g)药材以10 mL体积分数为90%的乙醇,在80 ℃下提取4 h。提取物中活性成分总含量和得率分别为0.53%,0.16%。结论：在文中较优提取条件下,玉屏风复方中活性成分群得到有效提取。

Investigation on one-pot extraction of active ingredient group from Yupingfeng powder by HPLC

The extraction of active ingredient group, i.e., prim-o-glucosylcimifugin, astragaloside, and 5-o-methylvisammiosode from Yupingfeng powder with one-pot method was studied in this work. A HPLC method was used to determine the content of each active constituent mentioned above in the extract. The influences of extraction temperature, time, volume percent of ethanol in water and its amount added on the content and yield of active ingredient group were investigated by orthogonal test. The experimental results showed that the optimized extraction conditions were as follows: 1g of Yupingfeng powder was one-pot extracted for 4 hours at 80 degrees C with 90% ethanol as solvent, and the yield and content of active ingredient group were 0.16%, 0.53% respectively. The active ingredient group in Yupingfeng powder could be effectively one-pot extracted under the conditions above.