Identification of amplified DNA sequences on double minute chromosomes in a leukemic cell line KY821 by means of spectral karyotyping and comparative genomic hybridization |
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| Authors: | Yoji Ariyama Tomoya Sakabe Takashi Shinomiya Toshiki Mori Yoji Fukuda J Inazawa |
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| Institution: | (1) Human Genome Center, Institute of Medical Science, The University of Tokyo, Shirokanedai 4-6-1, Tokyo 108, Japan Tel. +81-3-5449-5237 Fax +81-3-5449-5406 e-mail: johinaz@ims.u-tokyo.ac.jp, JP;(2) Third Department of Internal Medicine, Kinki University, School of Medicine, Osaka, Japan, JP |
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| Abstract: | Double minute chromosomes (dmin) are cytogenetic hallmarks of amplified genes. Using spectral karyotyping (SKY) and comparative
genomic hybridization (CGH), we identified the origin of amplified DNA in a leukemic cell line, KY821, that harbors numerous
dmin. The SKY revealed that the DNA sequences of dmin are derived from materials of chromosome 8, and CGH showed a high degree
of overrepresentation only at 8q22–24, indicating that in KY821 only chromosomal material of 8q22–24, containing MYC, is amplified in dmin. An approach combining SKY with CGH should facilitate efforts to identify novel chromosomal regions
of gene amplification and contribute information about genetic lesions that underly neoplastic tumors.
Received: March 11, 1998 / Accepted April 9, 1998 |
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| Keywords: | Leukemia cell line KY821 DNA amplification dmin CGH SKY |
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