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| 丙泊酚对肝癌细胞侵袭能力的影响 | |
| 引用本文： | 李培生,黄晓光,杨惠钿,林东红,林锦梅,林敏.丙泊酚对肝癌细胞侵袭能力的影响[J].分子诊断与治疗杂志,2012,0(6):385-388. |
| 作者姓名： | 李培生 黄晓光 杨惠钿 林东红 林锦梅 林敏 |
| 作者单位： | 1. 南方医科大学附属潮州市中心医院麻醉科,广东,潮州521021 2. 广州医学院检验系,广东,广州510182 3. 南方医科大学附属潮州市中心医院检验实验中心,广东,潮州521021 |
| 基金项目： | 广东省医学科学技术研究基金（B2008179）; 广东省社会发展计划（2011B031800329） |
| 摘 要： | 目的探讨丙泊酚对肝癌细胞株SMMC-7721侵袭特性和MMP-2、MMP-9的影响。方法不同浓度的丙泊酚处理SMMC-7721细胞,用Transwell小室模型测定SMMC-7721对细胞外基质Matrigel的侵袭力,明胶酶谱法检测培养上清液中MMP-2、MMP-9的变化。结果经过5μg/mL、10μg/mL、20μg/mL的丙泊酚处理后,SMMC-7721侵袭力均明显下调,培养上清液中MMP-2、MMP-9的含量也明显下降。结论丙泊酚可抑制肝癌细胞株SMMC-7721的体外侵袭力,同时明显降低SMMC-7721细胞分泌基质金属蛋白酶。 |
| 关 键 词： | 丙泊酚 肝癌细胞 侵袭 MMP-2 MMP-9 |
Anti-invasion effect of propofol on liver cancer cell |
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| LI Peisheng,HUANG Xiaoguang,YANG Huitian,LIN Donghong,LIN Jinmei,LIN Min.Anti-invasion effect of propofol on liver cancer cell[J].Journal of Molecular Diagnosis and Therapy,2012,0(6):385-388. | |
| Authors: | LI Peisheng HUANG Xiaoguang YANG Huitian LIN Donghong LIN Jinmei LIN Min |
| Institution: | 1.Anesthesiology Department, Chaozhou Central Hospital, Southern Medical University, Guangdong, Chaozhou 521021, China; 2.Inspection department, Guangzhou Medical University, Guangdong, Guangzhou 510182, China; 3.Central Laboratory, Chaozhou Central Hospital, Southern Medical University, Guangdong, Chaozhou 521021, China) |
| Abstract: | Objective To study the anti-invasion effect of propofol on liver cancer cell and expression of MMP-2 and MMP-9. Methods Propofol-treated SMMC-7721 invasion to matrigel were detected by transwell chamber method. The contents of MMP-2 and MMP-9 in culture supernatant were measured by gelatin zymography. Results Propofol inhibited SMMC-7721 invasion to the matrigel at the concentration of 5 μg/mL, 10 μg/mL and 20 μg/mL. Meanwhile, the concentrations of MMP-2 and MMP-9 were significantly decreased in matrix. Conclusion Propofol could significantly decrease the invasion effect and metalloproteinase expression of SMMC-7721. |
| Keywords: | Propofol Liver cancer cell Invasion MMP-2 MMP-9 |
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