四物合剂对人红白血病细胞株K562/ADM多药耐药性的逆转作用研究

目的 :观察四物合剂对人红白血病细胞株K5 62 /ADM多药耐药性的逆转作用。方法 :以维拉帕米为阳性对照 ,MTT法观察耐药细胞株K5 62 /ADM的耐药倍数及四物合剂的逆转倍数 ;采用反相高效液相色谱法 (RP-HPLC)检测细胞内的ADM浓度 ;免疫荧光法测定细胞膜P-糖蛋白 (Pgp)表达。结果 :四物合剂和ADM合用时 ,对K562/ADM耐药性的逆转倍数及细胞内的ADM含量比ADM单独使用时明显增高 (P<0.01);但对K562/ADM细胞膜Pgp表达的影响差异不显著 (P>0.05)。结论 :四物合剂在无毒性剂量时能逆转细胞株K562/ADM对ADM的耐药性 ,但对细胞膜Pgp表达影响不大 ,其逆转作用可能与降低Pgp药物外排作用、增加细胞内药物浓度有关。

Studies on the effects of Siwu Mixture on reversing multidrug resistance of human erythrocyte leukemic cell line K562/ADM

Objective: To study the effects of Siwu Mixture on reversing the multidrug resistance (MDR) of human erythrocyte leukemic cell line K562/ADM. Method: With verapamil set as a positive control, MTT assay was applied to evaluate the resistant factor of cell line K562/ADM to anticancer drugs and the reversal index of Siwu Mixture on MDR of the cells. The concentration of adriamycin(ADM) in the cells was measured by revers-phase high performance liquid chromatography. The immunofluorescence method was used to determine the expression of P-glucoprotein(Pgp) on cell membrane. Result: When ADM was combined with Siwu Mixture, its concentration in the cells and the reversal index of MDR were significantly higher than those of blank control(P<0.01). However, the expression of Pgp on cell membrane showed little difference (P>0.05). Conclusion: In case of noncytotoxic dose, Siwu Mixture showed significant effect on reversing MDR of cell line K562/ADM. The effect might be related to decreasing efflux of anticancer drugs in the cells and increasing their concentration in the cells. Nevertheless, it had no influence on the expression of Pgp on cell membrane.