银杏叶提取物对LPS诱导HeLa细胞凋亡的影响

目的研究银杏叶提取物（Ginkgo biloba leaf extract,GbE）对细菌脂多糖（LPS）诱导的人宫颈癌细胞HeLa细胞凋亡的影响。结论将HeLa细胞分成LPS组、GbE＋LPS干预组和对照组,各组细胞加入相应药物干预8～72h。采用MTT法检测GbE对细胞的毒性作用;计算各组细胞不同时间段细胞数,测定各组细胞的增殖情况;通过细胞形态学检测（AO/EB染色）测定细胞凋亡发生情况。结果在72h内不同浓度GbE对HeLa细胞无明显细胞毒性作用,细胞存活率在90%～100%之间,差异无统计学意义（P〉0.05）;LPS组经LPS诱导8h后细胞在各时间段增殖均较其他两组活跃,细胞数明显高于其他两组（tGbE＋LPS=6.423,P〈0.001;t对照组=3.976,P〈0.005）;GbE＋LPS组培养48h后细胞凋亡数明显高于LPS组和对照组。结论 GbE具有抑制LPS诱导的HeLa细胞增殖及促进细胞凋亡的作用。

Effects of Ginkgo biloba extract on LPS-induced HeLa cells apoptosis

Objective To investigate the effects of Ginkgo biloba extract （GbE） on lipopolysaccharides（LPS）- induced apoptosis in HeLa cells. Methods HeLa cells were assigned into three groups：LPS group,GbE＋LPS group and the control groups.Cells were pretreated with the corresponding drugs for 8-72 h.The cytotoxicity of GbE was evaluated by MTT assay. The morphology of HeLa cells and acridine orange/ethidium bromide （AO/EB） double staining methods were used to evaluate the tumour cell apoptosis. Results At the concentration used in this study,GbE was not cytotoxic to HeLa cells. The survival rate of the treated cells was between 90% and 100%（P0.05）.LPS could significantly induce cell proliferation at 8 h after treatment,and the number of HeLa cells was significantly higher than the other two groups （tGbE＋LPS=6.423,P0.001;tcontrol=3.976,P0.005）.The number of apoptotic cells in the GbE＋LPS group was significantly higher than the LPS group and the control group. Conclusion GbE can inhibit LPS-induced proliferation and promote apoptosis of HeLa cells.