银屑病患者外周血单个核细胞CCR5的表达及其意义

目的研究银屑病患者外周血单个核细胞趋化因子受体5（CCR5）的表达及其与银屑病皮损面积和严重程度指数（PASI）的关系。方法分离35例中重度寻常型银屑病患者及30例健康对照者外周血单个核细胞（PBMCs）,用RT-PCR法测定PBMCs培养前后CCR5 mRNA的表达水平,免疫荧光标记-流式细胞仪检测CCR5阳性细胞比率。结果治疗前银屑病患者外周血单个核细胞中CCR5 mRNA表达水平明显高于治疗后及健康对照组,并与PASI呈正相关（r=0.516,P〈0.05）。结论 CCR5可能通过活化与趋化单个核细胞到银屑病皮损而参与了银屑病的发病。

Expression of CCR5 in peripheral blood mononuclear cells in patients with psoriasis

Objective To investigate the expression of CCR5 in the peripheral blood mononuclear cells（PBMCs） in patients with psoriasis and the correlation between CCR5 expression and psoriasis area and severity index（PASI）.Methods PBMCs were isolated from 35 moderate to severe psoriasis patients and 30 health control.Expression of CCR5 mRNA and the rate of CCR5 positive cells on PBMC before and after culture was respectively detected by RT-PCR and immunofluorescence marker flow cytometry.Results CCR5 mRNA level in PBMCs from untreated psoriasis patients was higher than those in treated patients and normal controls.CCR5 mRNA expression was positively correlated with PASI（r=0.516,P〈0.05）.Conclusion Our study suggests that CCR5 may be involved in the pathogenesis of the progression of psoriasis by activating and aggregating mononuclear cells into psoriasis lesions.